Structure of PDB 5c71 Chain A

Receptor sequence
>5c71A (length=583) Species: 5062 (Aspergillus oryzae) [Search protein sequence]
MLKPQQTTTRDLISLDGLWKFALASDDNNTQPWTSQLKTSLECPVPASYN
DIFADSKIHDHVGWVYYQRDVIVPKGWSEERYLVRCEAATHHGRIYVNGN
LVADHVGGYTPFEADITDLVAAGEQFRLTIAVDNELTYQTIPPGKVEILE
ATGKKVQTYQHDFYNYAGLARSVWLYSVPQQHIQDITVRTDVQGTTGLID
YNVVASTTQGTIQVAVIDEDGTTVATSSGSNGTIHIPSVHLWQPGAAYLY
QLHASIIDSSKKTIDTYKLATGIRTVKVQGTQFLINDKPFYFTGFGKHED
TNIRGKGHDDAYMVHDFQLLHWMGANSFRTSHYPYAEEVMEYADRQGIVV
IDETPAVGLAFSPATFSPDRINNKTREAHAQAIRELIHRDKNHPSVVMWS
IANDPASNEDGAREYFAPLPKLARQLDPTRPVTFANVGLATYKADRIADL
FDVLCLNRYFGWYTQTAELDEAEAALEEELRGWTEKYDKPIVMTDYGADT
VAGLHSVMVTPWSEEFQVEMLDMYHRVFDRFEAMAGEQVWNFADFQTAVG
VSRVDGNKKGVFTRDRKPKAAAHLLRKRWTNLH
3D structure
PDB5c71 Structure-guided engineering of the substrate specificity of a fungal beta-glucuronidase toward triterpenoid saponins.
ChainA
Resolution2.62 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D162 H298 H332 V357 L359 D414 Y469 D505 K569
Catalytic site (residue number reindexed from 1) D162 H298 H332 V357 L359 D404 Y459 D495 K559
Enzyme Commision number 3.2.1.31: beta-glucuronidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CBW A D414 L449 Y469 Y473 D404 L439 Y459 Y463
BS02 GCU A D162 Y469 Y473 W550 R563 N567 K569 D162 Y459 Y463 W540 R553 N557 K559
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0004566 beta-glucuronidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0030246 carbohydrate binding
Biological Process
GO:0005975 carbohydrate metabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:5c71, PDBe:5c71, PDBj:5c71
PDBsum5c71
PubMed29146597
UniProtA7XS03

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