Structure of PDB 5c5h Chain A

Receptor sequence
>5c5hA (length=443) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
SHMIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGFAVQG
VVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNL
TLQFALVPDGENTFPALTSLHIQLVEGAHAATWQPTRLCSMTLTSSTGLP
KAAVHTYQAHLASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWKWLYA
GARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSLKAVLLGGAA
IPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVK
IVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVG
RLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKFGHRPVAVMEY
DHESVDLSEWVKDKLARFQQPVRWLTLPPKISRQALKEWVQRQ
3D structure
PDB5c5h Mechanism of MenE Inhibition by Acyl-Adenylate Analogues and Discovery of Novel Antibacterial Agents.
ChainA
Resolution2.401 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T142 S162 H186 G270 E273 F356 G361
Catalytic site (residue number reindexed from 1) T144 S163 H187 G271 E274 F357 G362
Enzyme Commision number 6.2.1.26: o-succinylbenzoate--CoA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A R9 C34 D38 Q74 R11 C36 D40 Q76
BS02 4YB A S188 L223 L244 G246 G247 A248 A249 Y269 G270 L271 T272 S276 T277 D336 I347 K437 S189 L224 L245 G247 G248 A249 A250 Y270 G271 L272 T273 S277 T278 D337 I348 K430 MOAD: Kd=394nM
PDBbind-CN: -logKd/Ki=6.40,Kd=394nM
BS03 MG A Q132 P133 T134 Q134 P135 T136
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008756 o-succinylbenzoate-CoA ligase activity
GO:0016874 ligase activity
GO:0016877 ligase activity, forming carbon-sulfur bonds
GO:0042802 identical protein binding
Biological Process
GO:0009234 menaquinone biosynthetic process
Cellular Component
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5c5h, PDBe:5c5h, PDBj:5c5h
PDBsum5c5h
PubMed26394156
UniProtP37353|MENE_ECOLI 2-succinylbenzoate--CoA ligase (Gene Name=menE)

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