Structure of PDB 5bus Chain A

Receptor sequence

>5busA (length=481) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

TEQPNWLMQRAQLTPERIALIYEDQTVTFAELFAASKRMAEQLAAHSVRK
GDTAAILLQNRAEMVYAVHACFLLGVKAVLLNTKLSTHERLFQLEDSGSG
FLLTDSSFEKKEYEHIVQTIDVDELMKEAAEEIEIEAYMQMDATATLMYT
SGTTGKPKGVQQTFGNHYFSAVSSALNLGITEQDRWLIALPLFHISGLSA
LFKSVIYGMTVVLHQRFSVSDVLHSINRHEVTMISAVQTMLASLLEETNR
CPESIRCILLGGGPAPLPLLEECREKGFPVFQSYGMTETCSQIVTLSPEF
SMEKLGSAGKPLFSCEIKIERDGQVCEPYEHGEIMVKGPNVMKSYFNRES
ANEASFQNGWLKTGDLGYLDNEGFLYVLDRRSDLIISGGENIYPAEVESV
LLSHPAVAEAGVSGAEDKKKVPHAYLVLHKPVSAGELTDYCKERLAKYKI
PAKFFVLDRLPRNASNKLLRNQLKDARKGEL

3D structure

• PDB: 5bus Structural Basis for the ATP-dependent Configuration of Adenylation Active Site in Bacillus subtilis o-Succinylbenzoyl-CoA Synthetase
• Chain: A
• Resolution: 2.603 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T152 S172 H196 T289 E290
• Catalytic site (residue number reindexed from 1): T150 S170 H194 T287 E288
• Enzyme Commision number: 6.2.1.26: o-succinylbenzoate--CoA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AMP	A	H196 G264 G265 S285 M288 T289 D367 V379 R382 K471	H194 G262 G263 S283 M286 T287 D365 V377 R380 K467

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008756 o-succinylbenzoate-CoA ligase activity
• GO:0016405 CoA-ligase activity
• GO:0016874 ligase activity

Biological Process

• GO:0009234 menaquinone biosynthetic process

External links

• PDB: RCSB:5bus, PDBe:5bus, PDBj:5bus
• PDBsum: 5bus
• PubMed: 26276389
• UniProt: P23971|MENE_BACSU 2-succinylbenzoate--CoA ligase (Gene Name=menE)