Structure of PDB 5b4f Chain A

Receptor sequence
>5b4fA (length=294) Species: 262724 (Thermus thermophilus HB27) [Search protein sequence]
VCKVCGQKAQVEMRSRGLALCREHYLDWFVKETERAIRRHRMLLPGERVL
VAVSGGKDSLALWDVLSRLGYQAVGLHIELGIGEYSKRSLEVTQAFARER
GLELLVVDLKEAYGFGVPELARLSGRVACSACGLSKRYIINQVAVEEGFR
VVATGHNLDDEAAVLFGNLLNPQGPVLPEKPGLAARVKPFYRFSEREVLS
YTLLRGIRYLHEECPNAKGAKSLLYKEALNLVERSMPGAKLRFLDGFLLR
ECERCGYPTTGAVCAFCRMWDAVYRRAKKRKLLPEEVSFRPRVK
3D structure
PDB5b4f Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA
ChainA
Resolution2.75 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.8.1.15: tRNA-5-methyluridine(54) 2-sulfurtransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A C3 C6 C22 H25 C2 C5 C21 H24
BS02 ZN A C274 C277 C286 C289 C252 C255 C264 C267
BS03 SF4 A C130 C133 K137 C222 N224 C129 C132 K136 C214 N216
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0046872 metal ion binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0002098 tRNA wobble uridine modification
GO:0002143 tRNA wobble position uridine thiolation
GO:0008033 tRNA processing
GO:0034227 tRNA thio-modification
Cellular Component
GO:0002144 cytosolic tRNA wobble base thiouridylase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5b4f, PDBe:5b4f, PDBj:5b4f
PDBsum5b4f
PubMed28439027
UniProtQ72LF3|TTUA_THET2 tRNA-5-methyluridine(54) 2-sulfurtransferase (Gene Name=ttuA)

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