Structure of PDB 5b2p Chain A

Receptor sequence
>5b2pA (length=1455) Species: 401614 (Francisella tularensis subsp. novicida U112) [Search protein sequence]
MNFKILPIAIDLGVKNTGVFSAFYQKGTSLERLDNKNGKVYELSKDSYTL
LMNNRTARRHQRRGIDRKQLVKRLFKLIWTEQLNLEWDKDTQQAISFLFN
RRGFSFITDGYSVKAILMDIFDDYNGEDDSYLKLATEQESKISEIYNKLM
QKILEFKLMKLCTDIKDDTLKEITSYEFELLADYLANYSESLKTQKFYNI
QEFLKRHATINDRILDTLLTDDLDIWNFNFELHHFVFAVNKIKSEMASGG
RHRSQYFQEITNVLDENNHQEGYLKNFCENLHNKKYSNLSVKNLVNLIGN
LSNLELKPLRKYFNDKIHAKADHWDEQKFTETYCHWILGEWRVGVKDQDK
KDGAKYSYKDLCNELKQKVTKAGLVDFLLELDPCRTIPPYLDNNNRKPPK
CQSLILNPKFLDNQYPNWQQYLQELKKLQSIQNYLDSFETDLKVLKSSKD
QPYFVEYKSSNQQIASGQRDYKDLDARILQFIFDRVKASDELLLNEIYFQ
AKKLKQESSKKLDEVIANSQLSQILKSQHTNGIFEQGTFLHLVCKYYKQR
QRARDSRLYIMPEYRYDKKLHKYNNTGRFDDDNQLLTYCNHKPRQKRYQL
LNDLAGVLQVSPNFLKDKIGSDDDLFISKWLVEHIRGFKKACEDSLKIQK
DNRGLLNHKINIARNTKGKCEKEIFNLICKIEGYKHGLAYELGVLLFGEP
NEASKPEFDRKIKKFNSIYSFAQIQQIAFAERKGNANTCAVCSADNAHRM
QQIKIILSAKAQRLPAIPTRIVDGAVKKMATILAKNIVDDNWQNIKQVLS
AKHQLHIPIITESNAFEFEPALADVKGKSLKDRRKKALERISPENIFKDK
NNRIKEFAEELDHIIPRTLNDEANLICVTGNRIFCLRDNYRSFINLTPQE
QKAFRHALFLADENPIKQAVIRAINNRNRTFVNGTQRYFAEVLANNIYLR
AKKENLNTDKISFDYFGIPTIGNGRGIAEIRQLYEKVDSDIQAYAKGDKP
QASYSHLIDAMLAFCIAADEHRNDGSIGLEIDKNYSLYPDIFSQIKITDN
EFSDKKLVRKKAIEGFNTHRQMTRDGIYAENYLPILIHKELNEVRKGYTW
KNSEEIKIFKGKKYDIQQLNNLVYCLKFVDKPISIDIQISTLEELRNILT
TNNIAATAEYYYINLKTQKLHEYYIENYNTALGYKKYSKEMEFLRSLAYR
SERVKIKSIDDVKQVLDKDSNFIIGKITLPFKKEWQRLYREWQNTTIKDD
YEFLKSFFNVKSITKLHKKVRKDFSLPISTNEGKFLVKRKTWDNNFIYQI
LNDSDSRADGTKPFIPAFDISKNEIVEAIIDSFTSKNIFWLPKNIELQKV
DNKNIFAIDTSKWFEVETPSDLRDIGIATIQYKIDNNSRPKVRVKLDYVI
DDDSKINYFMNHSLLKSRYPDKVLEILKQSTIIEFESSGFNKTIKEMLGM
KLAGI
3D structure
PDB5b2p Structure and Engineering of Francisella novicida Cas9
ChainA
Resolution1.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A Y48 T49 L51 M52 R55 T56 R58 R59 H60 R62 R63 I65 Q69 K72 R73 Q92 Q93 S96 F97 N100 R101 R102 G103 F104 G308 R310 Q329 E330 G331 Y332 L365 R369 F372 N373 D374 K375 H377 A378 H394 E399 R401 L450 D451 N452 N453 N454 R455 K456 P457 K459 S506 S507 K508 Y512 Q522 I523 S525 G526 Q527 R622 K640 Y641 N642 N643 R646 K660 R662 Q663 K664 R665 Y666 D671 Q717 K718 Q798 Q801 R807 N812 T813 R849 P854 D859 G860 K871 K912 K914 R1047 S1048 N1051 N1084 T1086 K1109 R1226 K1227 F1233 T1235 H1236 R1237 Q1238 M1239 T1240 I1244 Y1245 A1246 Y1329 N1331 Q1335 E1369 R1370 K1372 P1397 E1401 F1425 V1427 K1435 K1436 V1437 R1438 K1439 D1440 S1442 L1443 P1444 R1456 T1458 W1459 I1464 Q1466 S1499 S1502 I1505 Y48 T49 L51 M52 R55 T56 R58 R59 H60 R62 R63 I65 Q69 K72 R73 Q92 Q93 S96 F97 N100 R101 R102 G103 F104 G249 R251 Q270 E271 G272 Y273 L306 R310 F313 N314 D315 K316 H318 A319 H335 E340 R342 L391 D392 N393 N394 N395 R396 K397 P398 K400 S447 S448 K449 Y453 Q463 I464 S466 G467 Q468 R554 K572 Y573 N574 N575 R578 K592 R594 Q595 K596 R597 Y598 D603 Q649 K650 Q723 Q726 R732 N737 T738 R763 P768 D773 G774 K785 K826 K828 R891 S892 N895 N928 T930 K953 R1059 K1060 F1066 T1068 H1069 R1070 Q1071 M1072 T1073 I1077 Y1078 A1079 Y1162 N1164 Q1168 E1202 R1203 K1205 P1230 E1234 F1258 V1260 K1268 K1269 V1270 R1271 K1272 D1273 S1275 L1276 P1277 R1289 T1291 W1292 I1297 Q1299 S1332 S1335 I1338
BS02 dna A F106 K405 Y449 L450 D451 N452 N453 V545 K546 R622 Q677 N725 K789 Y794 G809 N810 M825 S844 A845 Q848 T855 R856 V858 R920 R1047 R1241 D1242 E1449 D1472 R1474 K1583 N1608 K1609 F106 K346 Y390 L391 D392 N393 N394 V486 K487 R554 Q609 N657 K714 Y719 G734 N735 M750 S758 A759 Q762 T769 R770 V772 R834 R891 R1074 D1075 E1282 D1305 R1307 K1416 N1441 K1442
BS03 dna A N1448 K1451 D1470 S1473 R1474 P1509 N1554 S1555 R1556 K1558 R1585 Y1586 E1603 N1281 K1284 D1303 S1306 R1307 P1342 N1387 S1388 R1389 K1391 R1418 Y1419 E1436
BS04 ZN A C460 C657 C814 C817 C401 C589 C739 C742
BS05 CA A D11 H1162 D11 H1006
BS06 CA A K1415 D1417 K1248 D1250
BS07 CA A E1231 N1234 I1497 D1498 S1499 E1064 N1067 I1330 D1331 S1332
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0051607 defense response to virus

View graph for
Molecular Function
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Biological Process
External links
PDB RCSB:5b2p, PDBe:5b2p, PDBj:5b2p
PDBsum5b2p
PubMed26875867
UniProtA0Q5Y3|CAS9_FRATN CRISPR-associated endonuclease Cas9 (Gene Name=cas9)

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