Structure of PDB 5b16 Chain A

Receptor sequence

>5b16A (length=722) Species: 9606 (Homo sapiens)

VWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAA
AAAAAAAAAAAAAALWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEA
IKPCRPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMF
AHAPLTNIPLCKVIRFNIDYTIHFIEEMMPENFCVKGLELFSLFLFRDIL
ELYDWNLKGPCCPRFHFMPRFVRFLPDGGKEVLSMHQILLYLLRCSKAAA
AAAAAAAAAAAAAAAATGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLI
GYTFQDRCLLQLAMTHPSHHLNFGMNPDHARNSLSNCGIRQPKYGDTPSR
INHNERLEFLGDAVVEFLTSVHLYYLFPSLEEGGLATYRTAIVQNQHLAM
LAKKLELDRFMLYAHGPDLCRESDLRHAMANCFQALIGAVYLEGSLEEAK
QLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFE
EAIGVIFTHVRLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEY
LFIHFPDHHEGHLTLLRSSLVNNRTQAKVAEELGMQEYAITNDKTKRPVA
LRTKTLADLLQSFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDP
KSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGC
GKGPSIQQAEMGAAMDALEKYN

3D structure

• PDB: 5b16 Structure of Human DROSHA
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 3.1.26.3: ribonuclease III.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	L987 F988 T998 Y999 A1002 I1003 Q1005 F1069 E1076 V1077 W1078	L376 F377 T387 Y388 A391 I392 Q394 F458 E465 V466 W467
BS02	peptide	A	E1112 A1113 I1114 V1116 E1193 D1233 E1235 Y1236 F1240 V1243	E501 A502 I503 V505 E582 D622 E624 Y625 F629 V632
BS03	ZN	A	H609 H680	H152 H216
BS04	ZN	A	C538 H549 H1026	C81 H92 H415

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004525 ribonuclease III activity

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing
• GO:0031054 pre-miRNA processing

External links

• PDB: RCSB:5b16, PDBe:5b16, PDBj:5b16
• PDBsum: 5b16
• PubMed: 26748718
• UniProt: Q9NRR4|RNC_HUMAN Ribonuclease 3 (Gene Name=DROSHA)