Structure of PDB 5aor Chain A

Receptor sequence

>5aorA (length=1009) Species: 7227 (Drosophila melanogaster)

LGEAYRPLEQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDD
YKYTPVGPEHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLV
RQLFHLNVIEPFSGTLKKDEQLKPYPVKLSPNLINKIDEVIKGLDLPVVN
PRNIKIELDGPPIPLIVNLSRIESSVIPWAPPQANWNTWHACNIDEGELA
TTSIDDLSMDYERSLRDRRQNDNEYRQFLEFREKLPIAAMRSEILTAIND
NPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGYANIYVTQPRRISAISV
AERVARERCEQLGDTVGYSVRFESVFPRPYGAILFCTVGVLLRKLEAGLR
GVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDLHVILMSATIDTTKFSK
YFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESRRKRKEVEDEEQL
LSEDKDEAEINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKS
KNIPGAILVFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQ
RKVFEPVPEGVTKIILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNL
TSYATVWASKTNLEQRKGRAGRVRPGFCFTLCSRARFQALEDNLTPEMFR
TPLHEMALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDAN
DELTPLGRLLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFS
LDIGQRRLANHQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDW
KGLQMSTMNVIWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDV
SLALLCLGLYPNICVHKEKRKVLTTESKAALLHKTSVNCSNLAVTFPYPF
FVFGEKIRTRAVSCKQLSMVSPLQVILFGSRKIDLAANNIVRVDNWLNFD
IEPELAAKIGALKPALEDLITVACDNPSDILRLEEPYAQLVKVVKDLCVK
SAGDFGLQR

3D structure

• PDB: 5aor Structure of the RNA Helicase Mle Reveals the Molecular Mechanisms for Uridine Specificity and RNA-ATP Coupling.
• Chain: A
• Resolution: 2.08 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	H196 R442 R443 R470 T486 G488 V489 R492 K493 V635 F637 G662 W663 A668 H691 S692 Q693 T717 N718 I719 R739 Y752 R861 L862 P863 F894 E896 S899 L900 Q953 Q966 K1033 T1034 T1058 R1059	H60 R293 R294 R321 T337 G339 V340 R343 K344 V486 F488 G513 W514 A519 H542 S543 Q544 T568 N569 I570 R590 Y603 R712 L713 P714 F745 E747 S750 L751 Q804 Q817 K884 T885 T909 R910
BS02	ADP	A	G410 C411 G412 K413 T414 T415 R452 T725 R771 V772	G261 C262 G263 K264 T265 T266 R303 T576 R622 V623
BS03	ALF	A	T409 K413 E508 S723 R768 R771	T260 K264 E359 S574 R619 R622

Gene Ontology

Molecular Function

• GO:0001069 regulatory region RNA binding
• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0003682 chromatin binding
• GO:0003690 double-stranded DNA binding
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0003725 double-stranded RNA binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0033679 3'-5' DNA/RNA helicase activity
• GO:0034458 3'-5' RNA helicase activity
• GO:0043138 3'-5' DNA helicase activity
• GO:0106222 lncRNA binding

Biological Process

• GO:0007549 sex-chromosome dosage compensation
• GO:0008340 determination of adult lifespan
• GO:0009047 dosage compensation by hyperactivation of X chromosome
• GO:0010468 regulation of gene expression
• GO:0031453 positive regulation of heterochromatin formation
• GO:0032508 DNA duplex unwinding
• GO:0042714 dosage compensation complex assembly
• GO:0045433 male courtship behavior, veined wing generated song production
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0048675 axon extension
• GO:0050684 regulation of mRNA processing
• GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
• GO:2000765 regulation of cytoplasmic translation

Cellular Component

• GO:0000228 nuclear chromosome
• GO:0000785 chromatin
• GO:0000805 X chromosome
• GO:0005634 nucleus
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0005829 cytosol
• GO:0016456 X chromosome located dosage compensation complex, transcription activating
• GO:0072487 MSL complex
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5aor, PDBe:5aor, PDBj:5aor
• PDBsum: 5aor
• PubMed: 26545078
• UniProt: P24785|MLE_DROME Dosage compensation regulator mle (Gene Name=mle)