Structure of PDB 5a8r Chain A

Receptor sequence

>5a8rA (length=548) Species: 145263 (Methanothermobacter marburgensis)

KKLFLKALKKKFEGEDPDEKYTNFYCFGGWEQSARKKEFTEYAKKAAEKR
GGIPFYNPDIGVPLGQRKLMAYRVSGTDAYVEGDDLHFVNNAAIQQMVDD
IKRTVIVGMDTAHAVLEKRLGVEVTPETINEYMEAINHALPGGAVVQEHM
VEVHPGLVEDCYAKIFTGDDNLADELDKRILIDINKEFPEEQAEQLKSYI
GNRTYQVNRVPTIVVRTCDGGTVSRWSAMQIGMSFISAYKLCAGEAAIAD
FSYAAKHADVIEMGTIMPARRARGPNEPGGVAFGTFADIVQTSRVSDDPA
NVSLEVIAGAAALYDQVWLGSYMSGGVGFTQYATAAYTDDILDDFVYYGM
EYVDDKYGICGTKPTMDVVRDISTEVTLYSLEQYEEYPTLLEDHFGGSQR
AAVAAAAAGCSTAFATGNSNAGINGWYLSQILHKEAHSRLGFYGYDLQDQ
CGASNSLSIRSDEGLIHELRGPNYPNYAMNVGHQPEYAGIAQAPHAARGD
AFCTNPLIKVAFADKDLAFDFTSPRKSIAAGALREFMPEGERDLIIPA

3D structure

• PDB: 5a8r Didehydroaspartate Modification in Methyl-Coenzyme M Reductase Catalyzing Methane Formation.
• Chain: A
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q150 Y335 G447 N483
• Catalytic site (residue number reindexed from 1): Q147 Y332 G444 N480
• Enzyme Commision number: 2.8.4.1: coenzyme-B sulfoethylthiotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	COM	A	Y335 F445 Y446	Y332 F442 Y443
BS02	TP7	A	R228 K259 H260	R225 K256 H257
BS03	F43	A	A147 V148 V149 Q150 M153 Q233 M236 A246	A144 V145 V146 Q147 M150 Q230 M233 A243
BS04	TP7	A	R273 M326 F332 F445 M482 N483	R270 M323 F329 F442 M479 N480
BS05	F43	A	V330 G331 F332 T333 Q334 Y335 G444 F445	V327 G328 F329 T330 Q331 Y332 G441 F442

Gene Ontology

Molecular Function

• GO:0016740 transferase activity
• GO:0046872 metal ion binding
• GO:0050524 coenzyme-B sulfoethylthiotransferase activity

Biological Process

• GO:0015948 methanogenesis

External links

• PDB: RCSB:5a8r, PDBe:5a8r, PDBj:5a8r
• PDBsum: 5a8r
• PubMed: 27467699
• UniProt: P58815|MCRX_METTM Methyl-coenzyme M reductase II subunit alpha (Gene Name=mrtA)