Structure of PDB 5a8k Chain A

Receptor sequence

>5a8kA (length=548) Species: 145261 (Methanothermobacter wolfeii)

ADKLFINALKKKFEESPEEKKTTFYTLGGWKQSERKTEFVNAGKEVAEKR
GIPQYNPDIGTPLGQRVLMPYQVSTTDTYVEGDDLHFVNNAAMQQLWDDI
RRTVIVGLNHAHAVIEKRLGKEVTPETITHYLETVNHAMPGAAVVQEHMV
ETHPALVADSYVKVFTGNDEIADEIDPAFVIDINKQFPEDQAETLKAEVG
DGIWQVVRIPTIVSRTCDGATTSRWSAMQIGMSMISAYKQAAGEAATGDF
AYAAKHAEVIHMGTYLPVRRARGENEPGGVPFGYLADICQSSRVNYDDPV
RVSLDVVATGAMLYDQIWLGSYMSGGVGFTQYATAAYTDNILDDFTYFGK
EYVEDKYGLCEAPNTMDTVLDIATEVTFYGLEQYEEYPALLEDQFGGSQR
AAVVAAAAGCSTAFATGNAQTGLSGWYLSMYLHKEQHSRLGFYGYDLQDQ
CGASNVFSIRGDEGLPLELRGPNYPNYAMNVGHQGEYAGISQAPHAARGD
AFVFNPLVKIAFADDNLVFDFTNVRGEFAKGALREFEPAGERALITPA

3D structure

• PDB: 5a8k Didehydroaspartate Modification in Methyl-Coenzyme M Reductase Catalyzing Methane Formation.
• Chain: A
• Resolution: 1.41 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Q147 Y333 G445
• Catalytic site (residue number reindexed from 1): Q146 Y332 G444
• Enzyme Commision number: 2.8.4.1: coenzyme-B sulfoethylthiotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	COM	A	Y333 F443 Y444	Y332 F442 Y443
BS02	TP7	A	R270 M324 F330 F443 M480 N481	R269 M323 F329 F442 M479 N480
BS03	CA	A	E190 E194	E189 E193
BS04	CA	A	P58 I60 T62	P57 I59 T61
BS05	CA	A	K11 F14	K10 F13
BS06	F43	A	V328 G329 F330 T331 Q332 Y333 G442 F443	V327 G328 F329 T330 Q331 Y332 G441 F442
BS07	TP7	A	R225 K256 H257	R224 K255 H256
BS08	F43	A	A144 V145 V146 Q147 M150 Q230 M233 A243	A143 V144 V145 Q146 M149 Q229 M232 A242

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding
• GO:0050524 coenzyme-B sulfoethylthiotransferase activity

Biological Process

• GO:0015948 methanogenesis

External links

• PDB: RCSB:5a8k, PDBe:5a8k, PDBj:5a8k
• PDBsum: 5a8k
• PubMed: 27467699
• UniProt: H7CHY1