Structure of PDB 5a5w Chain A

Receptor sequence

>5a5wA (length=245) Species: 28901 (Salmonella enterica)

MIIPALNLIDGTVVRLHQGDYARQRDYGNDPLPRLQDYAAQGAGVLHLVD
LTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRTEEDVAALLKAGVARVV
IGSTAVKSPDVVKGWFERFGAQALVLALDVRIDEHGTKQVAVSGWQENSG
VSLEQLVETYLPVGLKHVLCTDISRAGTLAGSNVSLYEEVCARYPQIAFQ
SSGGIGDIDDIAALRGTGVRGVIVGRALLEGKFTVKEAIQCWQNV

3D structure

• PDB: 5a5w Two-Step Ligand Binding in a Beta/Alpha8 Barrel Enzyme -Substrate-Bound Structures Shed New Light on the Catalytic Cycle of Hisa
• Chain: A
• Resolution: 1.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N7 D129
• Catalytic site (residue number reindexed from 1): N7 D129
• Enzyme Commision number: 5.3.1.16: 1-(5-phosphoribosyl)-5- [(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GUO	A	N7 G19 V49 L51 G80 G81 R83 V100 G102 S103 D129 G144 W145 G177 S202 G203 G204 I223 G225 R226	N7 G19 V49 L51 G80 G81 R83 V100 G102 S103 D129 G144 W145 G177 S202 G203 G204 I223 G225 R226

Gene Ontology

Molecular Function

• GO:0003949 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity
• GO:0016853 isomerase activity

Biological Process

• GO:0000105 L-histidine biosynthetic process
• GO:0000162 tryptophan biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5a5w, PDBe:5a5w, PDBj:5a5w
• PDBsum: 5a5w
• PubMed: 26294764
• UniProt: P10372|HIS4_SALTY 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (Gene Name=hisA)