Structure of PDB 5a57 Chain A

Receptor sequence

>5a57A (length=1107) Species: 170187 (Streptococcus pneumoniae TIGR4)

MEKETGPEVDDSKVTYDTIQSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQ
QFNQVFINNHRITPEVTYKKINETTAEYLMKLRDDAHLINAEMTVRLQVV
DNQLHFDVTKIVNHNQVTPGQKIDDESKLLSSISFLGNALVSVSSDQTGA
KFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLAAGVWSNS
QNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTK
ELPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIA
MNFGSQAQNPFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYA
DIGKRIGGVEDFKTLIEKAKKYGAHLGIHVNASETYPESKYFNEKILRKN
PDGSYSYGWNWLDQGINIDAAYDLAHGRLARWEDLKKKLGDGLDFIYVDV
WGNGQSGDNGAWATHVLAKEINKQGWRFAIEWGHGGEYDSTFHHWAADLT
YGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLLGGYSMKDFE
GWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYKW
TPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLT
PWNWDANGKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQG
KTEEQELTVKDGKITLDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFN
SGTLKHWTISGDASKAEIVKSQGANDMLRIQGNKEKVSLTQKLTGLKPNT
KYAVYVGVDNRSNAKASITVNTGEKEVTTYTNKSLALNYVKAYAHNTRRN
NATVDDTSYFQNMYAFFTTGADVSNVTLTLSREAGDEATYFDEIRTFENN
SSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEKHDP
YTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYR
VTFEYEAGSDNTYAFVVGKGEFQSQASNLEMHELPNTWTDSKKAKKATFL
VTGAETGDTWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITL
TGKMLTE

3D structure

• PDB: 5a57 Structural Analysis of a Family 101 Glycoside Hydrolase in Complex with Carbohydrates Reveals Insights Into its Mechanism.
• Chain: A
• Resolution: 1.46 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.97: endo-alpha-N-acetylgalactosaminidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D577 N579 D581 N583 D588	D262 N264 D266 N268 D273
BS02	CA	A	E703 D728 H1258	E388 D413 H943
BS03	CA	A	D1233 E1235 E1281 W1284 D1411	D918 E920 E966 W969 D1091
BS04	5G0	A	H657 D658 E699 W724 D764 V765 E796 W797	H342 D343 E384 W409 D449 V450 E481 W482
BS05	GAL	A	H657 D658 W724 W726 W867 D1254	H342 D343 W409 W411 W552 D939

Gene Ontology

Molecular Function

• GO:0030246 carbohydrate binding
• GO:0033926 endo-alpha-N-acetylgalactosaminidase activity

External links

• PDB: RCSB:5a57, PDBe:5a57, PDBj:5a57
• PDBsum: 5a57
• PubMed: 26304114
• UniProt: Q2MGH6|GH101_STRPN Endo-alpha-N-acetylgalactosaminidase (Gene Name=SP_0368)