Structure of PDB 4zu9 Chain A

Receptor sequence

>4zu9A (length=582) Species: 224324 (Aquifex aeolicus VF5)

MKYILFATAGHVDHGKTTLIKTLTGIDTDRLPEEKKRGLSIDIGFAYIDF
PDINTRLEIIDVPGHERFIKNAIAGICSASGLILVVDPNEGIMPQTIEHL
RVAKSFGIKHGIAVLTKMDKVDEELAHIAEEELIAFLEKEEMNMEIVKVS
AVTGQGIEDLKNSIKKLLESINNLNKHKPLRIFVDSAFVVKGYGTVLRGS
CFEGEVKEGDKVVVEPIGVISRVRKMQNHGVFVKKAVAGERIALNLPEVD
AKKVKRGFLILKPESYEKSNVLIVKTEIDLKPGKIYQVFFGMRETVGKIS
VIDKGIYLVRLKENAIVRRGDKLVVLDSSGNFLGGAEVLHPKVRVTKKAF
IKKNIKDLLENFECYLLKERGPIGLKLEFFKRITGVSPKVANLKPESIEI
RGVYYLKGFIENLKLKIKKFLDTELQNAFGVDKEKVKSMFSLNEELLKYI
LDELKTYKIVNELIIDERKSDLEKNEDFQKLMSILKGGIKEEREIILEGI
PKEILTLSIKRKYAHRIGEYLIISDELLKKYINELKELGKTFNVQQAKNK
LGLTRKYLIPLLEYLDYLGLTVREGNERRWKR

3D structure

• PDB: 4zu9 Crystal structure of the full-length bacterial selenocysteine-specific elongation factor SelB
• Chain: A
• Resolution: 3.191 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D13 K16 T17 S40 H65
• Catalytic site (residue number reindexed from 1): D13 K16 T17 S40 H65

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GNP	A	V12 D13 G15 K16 T17 T18 S40 G64 K117 D119 A151 V152	V12 D13 G15 K16 T17 T18 S40 G64 K117 D119 A151 V152
BS02	MG	A	T17 S40	T17 S40
BS03	CYS	A	R30 R198 H229 R241	R30 R198 H229 R241

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0001514 selenocysteine incorporation
• GO:0006414 translational elongation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4zu9, PDBe:4zu9, PDBj:4zu9
• PDBsum: 4zu9
• PubMed: 26304550
• UniProt: O67141