Structure of PDB 4zr5 Chain A

Receptor sequence
>4zr5A (length=696) Species: 9986 (Oryctolagus cuniculus) [Search protein sequence]
GICKSSDCIKSAARLIQNMDATAEPCTDFFKYACGGWLKRNVIPETSSRY
SNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCVNETAIDSRGG
QPLLKLLPDVYGWPVATQNWEQTYGTSWSAEKSIAQLNSKYGKKVLINFF
VGTDDKNSMNHIIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMIAVAK
LIRQEEGLPIDENQISVEMNKVMELEKEIANATTKSEDRNDPMLLYNKMT
LAQIQNNFSLEINGKPFSWSNFTNEIMSTVNINIPNEEDVVVYAPEYLIK
LKPILTKYSPRDLQNLMSWRFIMDLVSSLSRTYKDSRNAFRKALYGTTSE
SATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQT
LDDLTWMDAETKKKAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKE
DEYFENIIQNLKFSQSKQLKKLREKVDKDEWITGAAIVNAFYSSGRNQIV
FPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLV
DWWTQQSANNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGG
IGQAYRAYQNYVKKNGEEKLLPGIDLNHKQLFFLNFAQVWCGTYRPEYAV
NSIKTDVHSPGNFRIIGSLQNSVEFSEAFQCPKNSYMNPEKKCRVW
3D structure
PDB4zr5 Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan.
ChainA
Resolution2.8016 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H584 E585 H588 E647 D651 H712 R718
Catalytic site (residue number reindexed from 1) H530 E531 H534 E593 D597 H658 R664
Enzyme Commision number 3.4.24.11: neprilysin.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A H584 H588 E647 H530 H534 E593
BS02 RDF A R103 F107 D108 N543 A544 F545 V581 H584 E585 E647 W694 H712 R718 R49 F53 D54 N489 A490 F491 V527 H530 E531 E593 W640 H658 R664 BindingDB: IC50=6nM
Gene Ontology
Molecular Function
GO:0004222 metalloendopeptidase activity
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0042277 peptide binding
GO:0046872 metal ion binding
Biological Process
GO:0001822 kidney development
GO:0006508 proteolysis
GO:0006518 peptide metabolic process
GO:0010814 substance P catabolic process
GO:0010815 bradykinin catabolic process
GO:0016485 protein processing
GO:0019233 sensory perception of pain
GO:0042447 hormone catabolic process
GO:0046449 creatinine metabolic process
GO:0050435 amyloid-beta metabolic process
GO:0071345 cellular response to cytokine stimulus
GO:0071492 cellular response to UV-A
GO:0071493 cellular response to UV-B
GO:0090399 replicative senescence
GO:0097242 amyloid-beta clearance
Cellular Component
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0005903 brush border
GO:0008021 synaptic vesicle
GO:0030424 axon
GO:0030425 dendrite
GO:0044306 neuron projection terminus
GO:0045202 synapse

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4zr5, PDBe:4zr5, PDBj:4zr5
PDBsum4zr5
PubMed31204686
UniProtP08049|NEP_RABIT Neprilysin (Gene Name=MME)

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