Structure of PDB 4zi6 Chain A

Receptor sequence

>4zi6A (length=484) Species: 85962 (Helicobacter pylori 26695)

MLKIKLEKTTFENAKAECSLVFIINKDFSHAWVKNKELLETFKYEGEGVF
LDQENKILYAGVKEDDVHLLRESACLAVRTLKKLAFKSVKVGVYTCGANA
LLENLKALFLGLKLGLYEYDTFKSNKKESVLKEAIVALELHKSLEKSAKE
ALKYAEIMTESLNIVKDLVNTPPMIGTPVYMAEVAQKVAKENHLEIHVHD
EKFLEEKKMNAFLAVNKASLSVNPPRLIHLVYKPKKAKKKIALVGKGLTY
DCGGLSLKPADYMVTMKADKGGGSAVIGLLNALAKLGVEAEVHGIIGATE
NMIGPAAYKPDDILISKEGKSIEVRNTDAEGRLVLADCLSYAQDLNPDVI
VDFATLTGACVVGLGEFTSAIMGHNEELKNLFETSGLESGELLAKLPFNR
HLKKLIESKIADVCNISSSRYGGAITAGLFLNEFIRDEFKDKWLHIDIAG
PAYVEKEWDVNSFGASGAGVRACTAFVEELLKKA

3D structure

• PDB: 4zi6 Structural basis for substrate specificity of Helicobacter pylori M17 aminopeptidase.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K270 R344
• Catalytic site (residue number reindexed from 1): K258 R332
• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.11.10: bacterial leucyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	K258 D263 D281 E342	K246 D251 D269 E330
BS02	ZN	A	D263 D340 E342	D251 D328 E330
BS03	BCT	A	A341 E342 G343 R344 L368	A329 E330 G331 R332 L356
BS04	NA	A	A461 G462 Y465	A449 G450 Y453

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4zi6, PDBe:4zi6, PDBj:4zi6
• PDBsum: 4zi6
• PubMed: 26616008
• UniProt: O25294|AMPA_HELPY Cytosol aminopeptidase (Gene Name=pepA)