Structure of PDB 4yrt Chain A

Receptor sequence
>4yrtA (length=414) Species: 353153 (Trypanosoma cruzi strain CL Brener) [Search protein sequence]
MVETEPVQGCRDFPPEAMRCRRHLFDVFHATAKTFGFEEYDAPVLESEEL
YIRKAGEEITEQMFNFITKGGHRVALRPEMTPSLARLLLGKGRSLLLPAK
WYSIPQCWRYERRREHYQWNMDIVGVKSVSAEVELVCAACWAMRSLGLSS
KDVGIKVNSRKVLQTVVEQAGVTSDKFAPVCVIVDKMEKIPREEVEAQLA
VLGLEPTVVDAITTTLSLKSIDEIAQRVGEEHEAVKELRQFFEQVEAYGY
GDWVLFDASVVRGLAYYTGIVFEGFDREGKFRALCGGGRYDNLLTTYGSP
TPIPCAGFGFGDCVIVELLQEKRLLPDIPHVVDDVVIPFDESMRPHALAV
LRRLRDAGRSADIILDKKKVVQAFNYADRVGAVRAVLVAPEEWERGEVQV
KMLRGFAVPLDRLV
3D structure
PDB4yrt A binding hotspot in Trypanosoma cruzi histidyl-tRNA synthetase revealed by fragment-based crystallographic cocktail screens.
ChainA
Resolution2.05 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.21: histidine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HIS A T128 D174 L316 Y318 Y319 G359 F360 G361 T81 D122 L264 Y266 Y267 G307 F308 G309
BS02 781 A R156 R166 E167 H168 C365 V366 R109 R114 E115 H116 C313 V314 PDBbind-CN: -logKd/Ki=2.78,IC50=1.65mM
Gene Ontology
Molecular Function
GO:0004821 histidine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
Biological Process
GO:0006427 histidyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4yrt, PDBe:4yrt, PDBj:4yrt
PDBsum4yrt
PubMed26249349
UniProtQ4DA54

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