Structure of PDB 4yrc Chain A

Receptor sequence
>4yrcA (length=419) Species: 353153 (Trypanosoma cruzi strain CL Brener) [Search protein sequence]
NMVETEPVQGCRDFPPEAMRCRRHLFDVFHATAKTFGFEEYDAPVLESEE
LYIRKAGEEITEQMFNFITKGGHRVALRPEMTPSLARLLLGKGRSLLLPA
KWYSIPQCWRYEAITRRREHYQWNMDIVGVKSVSAEVELVCAACWAMRSL
GLSSKDVGIKVNSRKVLQTVVEQAGVTSDKFAPVCVIVDKMEKIPREEVE
AQLAVLGLEPTVVDAITTTLSLKSIDEIAQRVGEEHEAVKELRQFFEQVE
AYGYGDWVLFDASVVRGLAYYTGIVFEGFDREGKFRALCGGGRYDNLLTT
YGSPTPIPCAGFGFGDCVIVELLQEKRLLPDIPHVVDDVVIPFDESMRPH
ALAVLRRLRDAGRSADIILDKKKVVQAFNYADRVGAVRAVLVAPEEWERG
EVQVKMLRRGFAVPLDRLV
3D structure
PDB4yrc A binding hotspot in Trypanosoma cruzi histidyl-tRNA synthetase revealed by fragment-based crystallographic cocktail screens.
ChainA
Resolution2.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.21: histidine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HIS A T128 Q170 D174 L316 Y318 Y319 G359 F360 G361 T82 Q122 D126 L268 Y270 Y271 G311 F312 G313
BS02 0P6 A P125 C154 W155 R156 E167 H168 P79 C108 W109 R110 E119 H120 PDBbind-CN: -logKd/Ki=2.70,IC50>2mM
BS03 0P6 A V383 G433 V335 G385 PDBbind-CN: -logKd/Ki=2.70,IC50>2mM
Gene Ontology
Molecular Function
GO:0004821 histidine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
Biological Process
GO:0006427 histidyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4yrc, PDBe:4yrc, PDBj:4yrc
PDBsum4yrc
PubMed26249349
UniProtQ4DA54

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