Structure of PDB 4ypf Chain A

Receptor sequence
>4ypfA (length=406) Species: 353153 (Trypanosoma cruzi strain CL Brener) [Search protein sequence]
MVETEPVQGCRDFPPEAMRCRRHLFDVFHATAKTFGFEEYDAPVLESEEL
YIRKAGEEITEQMFNFITKGGHRVALRPEMTPSLARLLLGKGRSLLLPAK
WYSIPQCWRYERRREHYQWNMDIVGVKSVSAEVELVCAACWAMRSLGLSS
KDVGIKVNSRKVLQTVVEQAGVTSDKFAPVCVIVDKMEREEVEAQLAVTV
VDAITTTLSLKSIDEIAQRVGEEHEAVKELRQFFEQVEAYGYGDWVLFDA
SVVRGLAYYTGIVFEGFDREGKFRALCGGGRYDNLLTTYGSPTPIPCAGF
GFGDCVIVELLQEKRLLPDIPHVVDDVVIPFDESMRPHALAVLRRLRDAG
RSADIILDKKKVVQAFNYADRVGAVRAVLVAPEEWERGEVQVKMLRGFAV
PLDRLV
3D structure
PDB4ypf A binding hotspot in Trypanosoma cruzi histidyl-tRNA synthetase revealed by fragment-based crystallographic cocktail screens.
ChainA
Resolution2.2 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.21: histidine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HIS A T128 D174 L316 Y318 Y319 G359 F360 T81 D122 L256 Y258 Y259 G299 F300
BS02 4FS A P125 W155 R156 E167 H168 P78 W108 R109 E115 H116 PDBbind-CN: -logKd/Ki=2.70,IC50>2mM
Gene Ontology
Molecular Function
GO:0004821 histidine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
Biological Process
GO:0006427 histidyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4ypf, PDBe:4ypf, PDBj:4ypf
PDBsum4ypf
PubMed26249349
UniProtQ4DA54

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