Structure of PDB 4ykg Chain A

Receptor sequence

>4ykgA (length=521) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIAELSDKV
TFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGH
PSKEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIK
HTAIDGGTFQNEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAE
KRAAEELNKRDAYDVLIVGSGPAGAAAAIYSARKGIRTGLMGERFGGQIL
DTVDIENYISVPKTEGQKLAGALKVHVDEYDVDVIDSQSASKLIPAAVEG
GLHQIETASGAVLKARSIIVATGAKWRNMNVPGEDQYRTKGVTYCPHCDG
PLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKADQVLQDKL
RSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIA
TGEGAKASLSAFDYLIRTKTA

3D structure

PDB

4ykg Crystallographic and solution studies of NAD(+)- and NADH-bound alkylhydroperoxide reductase subunit F (AhpF) from Escherichia coli provide insight into sequential enzymatic steps

Chain

Resolution

2.4 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	C345 C348 D349
Catalytic site (residue number reindexed from 1)	C345 C348 D349
Enzyme Commision number	1.8.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	G221 P222 A223 G242 E243 G247 Q248 T252 N257 S289 A290 T322 G323 C348 G487 D488 Q496 I497	G221 P222 A223 G242 E243 G247 Q248 T252 N257 S289 A290 T322 G323 C348 G487 D488 Q496 I497
BS02	NAD	A	I361 G364 S366 E385 F386 I449 M467 P493	I361 G364 S366 E385 F386 I449 M467 P493

Gene Ontology

	Molecular Function
GO:0004791	thioredoxin-disulfide reductase (NADPH) activity
GO:0008785	alkyl hydroperoxide reductase activity
GO:0016491	oxidoreductase activity
GO:0016668	oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0050660	flavin adenine dinucleotide binding
GO:0051287	NAD binding
GO:0071949	FAD binding

	Biological Process
GO:0000302	response to reactive oxygen species
GO:0006979	response to oxidative stress
GO:0042744	hydrogen peroxide catabolic process
GO:0045454	cell redox homeostasis
GO:0098869	cellular oxidant detoxification

	Cellular Component
GO:0005829	cytosol
GO:0009321	alkyl hydroperoxide reductase complex

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:4ykg, PDBe:4ykg, PDBj:4ykg
PDBsum	4ykg
PubMed	26092085
UniProt	P35340\|AHPF_ECOLI Alkyl hydroperoxide reductase subunit F (Gene Name=ahpF)

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