Structure of PDB 4xxv Chain A

Receptor sequence

>4xxvA (length=356) Species: 271848 (Burkholderia thailandensis E264)

HMKIAVLPGDGIGPEIVNEAVKVLNALDEKFELEHAPVGGAGYEASGHPL
PDATLALAKEADAILFGAVGDWKYDSLERALRPEQAILGLRKHLELFANF
RPAICYPQLVDASPLKPELVAGLDILIVRELNGDIYFGQPRGVRAAPDGP
FAGEREGFDTMRYSEPEVRRIAHVAFQAAQKRAKKLLSVDKSNVLETSQF
WRDVMIDVSKEYADVELSHMYVDNAAMQLAKAPKQFDVIVTGNMFGDILS
DEASMLTGSIGMLPSASLDKNNKGLYEPSHGSAPDIAGKGIANPLATILS
AAMLLRYSLNRAEQADRIERAVKTVLEQGYRTGDIATPGCRQVGTAAMGD
AVVAAL

3D structure

• PDB: 4xxv Crystal structure of 3-isopropylmalate dehydrogenase from Burkholderia thailandensis in complex with NAD
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y135 K190 D222 D246 D250
• Catalytic site (residue number reindexed from 1): Y136 K191 D223 D247 D251
• Enzyme Commision number: 1.1.1.85: 3-isopropylmalate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	N192 Y220 N223	N193 Y221 N224

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003862 3-isopropylmalate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0046872 metal ion binding
• GO:0051287 NAD binding

Biological Process

• GO:0009098 L-leucine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:4xxv, PDBe:4xxv, PDBj:4xxv
• PDBsum: 4xxv
• UniProt: Q2T7H6|LEU3_BURTA 3-isopropylmalate dehydrogenase (Gene Name=leuB)