Structure of PDB 4xww Chain A

Receptor sequence

>4xwwA (length=545) Species: 1299 (Deinococcus radiodurans)

PAPTLEVIPLGGMGEIGKNITVFRYGDEIVVVDGGLAFPKAHQMGIDLIV
PRIDYLLEHQDKIKGWILTHGHEDHIGGLPYIFARLPRVPVYGLPLTLAL
VREKLSEFGLQDVDLREVTYGDEVRFGQSFVAEFFCMTHSIPDNAGYILK
TPVGDVLHTGDFKIDPDVGTGAGIVSDLERVEQAGKDGVLLLISDSTNAE
RPGHTPSEAEIARNLEEIIKGCRGRVFLTTFASQVYRIQNILDLAHRQGR
RVVMEGRSMIKYAQAAQATGHMNPPEPFLTSEEVGELQDQQVLFVCTGSQ
GQPMAVLGRLAFGTHAKIALRRGDTVILSSNPIPGNEDAVNLIVNRLYEI
GVDVVYPPTYRVHASGHASQEELATILNLTRPKFFLPWHGEPRHQINHAK
LAQTLPRPPKRTLIAKNGDIVNLGPDEFRVSGTVAAGAVYVDGLGVGDVN
DDVLLDRVNLSQEGLLILTAVLHPTPHVEVVARGFARPNRDLELQIRRVA
LEAVEQGLREKKRLEDVRDDMYGAVRRFTRKATGRNPVLIPMIVD

3D structure

• PDB: 4xww Structural insights into catalysis and dimerization enhanced exonuclease activity of RNase J
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	I30 F52 H86 E87 D88 A246 S247 R271 S272 T311 Q316 A319 R323 I347 P348 H377 S379 H381	I16 F38 H72 E73 D74 A232 S233 R257 S258 T297 Q302 A305 R309 I333 P334 H363 S365 H367
BS02	ZN	A	H84 H86 H153	H70 H72 H139
BS03	ZN	A	D88 H89 H403	D74 H75 H389
BS04	MN	A	G59 D456	G45 D442

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4xww, PDBe:4xww, PDBj:4xww
• PDBsum: 4xww
• PubMed: 25940620
• UniProt: H9CZL7