Structure of PDB 4xus Chain A

Receptor sequence

>4xusA (length=323) Species: 9606 (Homo sapiens)

HNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACS
IPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM
WYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQK
AAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSL
RQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFAC
ALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNHGCKVGPGRVLP
TPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 4xus Nucleotide binding interactions modulate dNTP selectivity and facilitate 8-oxo-dGTP incorporation by DNA polymerase lambda.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D427 D429 D490
• Catalytic site (residue number reindexed from 1): D176 D178 D239
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 R275 G278 Y279 P303 G304 G306 R308 M309 K312	W23 R24 G27 Y28 P52 G53 G55 R57 M58 K61
BS02	dna	A	W342 G343 G345 K347 T348	W91 G92 G94 K96 T97
BS03	dna	A	W274 V462 S463 Q464 Q471 K472 R514 R517 K521 L527 S528 H530 H541	W23 V211 S212 Q213 Q220 K221 R263 R266 K270 L276 S277 H279 H289
BS04	TTP	A	R386 G416 S417 R420 G426 D427 D429 Y505 T507 G508 A510 N513	R135 G165 S166 R169 G175 D176 D178 Y254 T256 G257 A259 N262
BS05	MG	A	S339 I341 A344	S88 I90 A93
BS06	MG	A	D427 D429	D176 D178
BS07	DOC	A	D490 Y505	D239 Y254

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4xus, PDBe:4xus, PDBj:4xus
• PDBsum: 4xus
• PubMed: 26220180
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)