Structure of PDB 4xbo Chain A

Receptor sequence
>4xboA (length=228) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALA
AGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKS
VAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQ
VIAYEVRMAWLATQTPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMN
KLRRLFTRARPESQELNILRGILASIEQ
3D structure
PDB4xbo tRNA recognition by a bacterial tRNA Xm32 modification enzyme from the SPOUT methyltransferase superfamily
ChainA
Resolution2.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.200: tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A T79 S80 A81 G114 R115 I134 S142 L143 A148 T79 S80 A81 G114 R115 I134 S142 L143 A148 PDBbind-CN: -logKd/Ki=4.59,Kd=26uM
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0008168 methyltransferase activity
GO:0008173 RNA methyltransferase activity
GO:0042803 protein homodimerization activity
Biological Process
GO:0002128 tRNA nucleoside ribose methylation
GO:0006396 RNA processing
GO:0008033 tRNA processing
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Cellular Component
External links
PDB RCSB:4xbo, PDBe:4xbo, PDBj:4xbo
PDBsum4xbo
PubMed26202969
UniProtP0AE01|TRMJ_ECOLI tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ (Gene Name=trmJ)

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