Structure of PDB 4x0q Chain A

Receptor sequence

>4x0qA (length=625) Species: 9606 (Homo sapiens)

SLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRDDTLVVGLAVCWG
GRDAYYFSLLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILL
LSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMET
SQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRK
VEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSF
SFTSSDDIAEVLFLELKLPPQFSTSKDVLNKLKALHPLPGLILEWRRITN
AITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRD
FEIKMGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQV
LNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQ
MGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLP
GIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKS
HGHREGMLQSDCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESA
VKLSVKLKVKVKIGASWGELKDFDV

3D structure

• PDB: 4x0q Human DNA polymerase theta grasps the primer terminus to mediate DNA repair.
• Chain: A
• Resolution: 3.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.49: RNA-directed DNA polymerase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	K2209 A2238 T2239 T2245 N2248 G2388 M2393 G2394 A2462 H2463 R2466 Q2467 N2470 Q2474	K304 A333 T334 T340 N343 G437 M442 G443 A511 H512 R515 Q516 N519 Q523
BS02	dna	A	S2180 K2181 R2202 R2241 N2251 R2254 L2538 H2539	S275 K276 R297 R336 N346 R349 L573 H574
BS03	dna	A	N2457 P2458	N506 P507
BS04	MG	A	D2330 D2540	D379 D575
BS05	DG3	A	K2383 Q2384 Y2387 D2540	K432 Q433 Y436 D575

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:4x0q, PDBe:4x0q, PDBj:4x0q
• PDBsum: 4x0q
• PubMed: 25775267
• UniProt: O75417|DPOLQ_HUMAN DNA polymerase theta (Gene Name=POLQ)