Structure of PDB 4wyc Chain A

Receptor sequence

>4wycA (length=428) Species: 83332 (Mycobacterium tuberculosis H37Rv)

LTPEQIIAVDGAHLWHPYSSIGREAVSPVVAVAAHGAWLTLIRDGQPIEV
LDAMSSWWTAIHGHGHPALDQALTTQLRVMNHVMFGGLTHEPAARLAKLL
VDITPAGLDTVFFSDSGSVSVEVAAKMALQYWRGRGLPGKRRLMTWRGGY
HGDTFLAMSICDPHGGMHSLWTDVLAAQVFAPQVPRDYDPAYSAAFEAQL
AQHAGELAAVVVEPVVQGAGGMRFHDPRYLHDLRDICRRYEVLLIFDEIA
TGFGRTGALFAADHAGVSPDIMCVGKALTGGYLSLAATLCTADVAHTISA
GAAGALMHGPTFMANPLACAVSVASVELLLGQDWRTRITELAAGLTAGLD
TARALPAVTDVRVCGAIGVIECDRPVDLAVATPAALDRGVWLRPFRNLVY
AMPPYICTPAEITQITSAMVEVARLVGS

3D structure

• PDB: 4wyc Fragment-Based Exploration of Binding Site Flexibility in Mycobacterium tuberculosis BioA.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y25 Y157 E220 D254 A257 K283 Y407
• Catalytic site (residue number reindexed from 1): Y18 Y150 E213 D247 A250 K276 Y400
• Enzyme Commision number: 2.6.1.62: adenosylmethionine--8-amino-7-oxononanoate transaminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	A	G124 S125 Y157 H158 G159 E220 D254 I256 K283	G117 S118 Y150 H151 G152 E213 D247 I249 K276
BS02	3VS	A	P24 Y25 W64 Y157	P17 Y18 W57 Y150	MOAD: Kd=15uM
BS03	PLP	A	P317 T318	P310 T311
BS04	3VS	A	M91 G93 G316	M84 G86 G309	MOAD: Kd=15uM

Gene Ontology

Molecular Function

• GO:0004015 adenosylmethionine-8-amino-7-oxononanoate transaminase activity
• GO:0008483 transaminase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0009102 biotin biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4wyc, PDBe:4wyc, PDBj:4wyc
• PDBsum: 4wyc
• PubMed: 26068403
• UniProt: P9WQ81|BIOA_MYCTU Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (Gene Name=bioA)