Structure of PDB 4wuo Chain A

Receptor sequence
>4wuoA (length=349) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence]
ASMKVAVLPGDGIGPEVTEAALKVLRALDEAEGLGLAYEVFPFGGAAIDA
FGEPFPEPTRKGVEEAEAVLLGSVGGPKWDGLPRKIRPETGLLSLRKSQD
LFANLRPAKVFPGLERLSPLKEEIARGVDVLIVRELTGGIYFGEPRGMSE
AEAWNTERYSKPEVERVARVAFEAARKRRKHVVSVDKANVLEVGEFWRKT
VEEVGRGYPDVALEHQYVDAMAMHLVRSPARFDVVVTGNIFGDILSDLAS
VLPGSLGLLPSASLGRGTPVFAPVHGSAPDIAGKGIANPTAAILSAAMML
EHAFGLVELARKVEDAVAKALLETPPPDLGGSAGTEAFTATVLRHLAAA
3D structure
PDB4wuo Glutamate 270 plays an essential role in K(+)-activation and domain closure of Thermus thermophilus isopropylmalate dehydrogenase.
ChainA
Resolution2.05 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y139 K185 D217 D241 D245
Catalytic site (residue number reindexed from 1) Y141 K187 D219 D243 D247
Enzyme Commision number 1.1.1.85: 3-isopropylmalate dehydrogenase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 IPM A R94 R104 R132 Y139 D241 R96 R106 R134 Y141 D243
BS02 NAD A I11 S71 V72 G73 E87 L90 G255 H273 G274 S275 A276 D278 I279 N286 I13 S73 V74 G75 E89 L92 G257 H275 G276 S277 A278 D280 I281 N288
BS03 NAD A Y215 M221 Y217 M223
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003862 3-isopropylmalate dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0042802 identical protein binding
GO:0046872 metal ion binding
GO:0051287 NAD binding
Biological Process
GO:0009098 L-leucine biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4wuo, PDBe:4wuo, PDBj:4wuo
PDBsum4wuo
PubMed25497013
UniProtQ5SIY4|LEU3_THET8 3-isopropylmalate dehydrogenase (Gene Name=leuB)

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