Structure of PDB 4wtf Chain A

Receptor sequence

>4wtfA (length=536) Species: 356411 (Hepatitis C virus JFH-1)

SSMSYSWTGALITPCGPEEEKLPINPLSNSLLRYHNKVYCTTSKSASQRA
KKVTFDRTQVLDAHYDSVLKDIKLAASKVSARLLTLQQACQLTPPHSARS
KYGFGAKEVRSLSGRAVNHIKSVWKDLLEDPQTPIPTTIMAKNEVFCVDP
AKGGKKPARLIVYPDLGVRVCEKMALYDITQKLPQAVMGASYGFQYSPAQ
RVEYLLKAWAEKKDPMGFSYDTRHFDSTVTERDIRTEESIYQACSLPEEA
RTAIHSLTERLYVGGPMFNSKGQTCGYRRCRASGVLTTSMGNTITCYVKA
LAACKAAGIVAPTMLVCGDDLIVISESQGTEEDERNLRAFTEAMTRYSAP
PGDPPRPEYDLELITSCSSNVSVALGPRGRRRYYLTRDPTTPLARAAWET
VRHSPINSWLGNIIQYAPTIWVRMVLMTHFFSILMVQDTLDQNLGGVNPL
DLPAIIERLHGLDAFSMHTYSHHELTRVASALRKLGAPPLRVWKSRARAV
RASLISRGGKAAVCGRYLFNWAVKTKLKLTPLPEAR

3D structure

• PDB: 4wtf Structural basis for RNA replication by the hepatitis C virus polymerase.
• Chain: A
• Resolution: 2.65 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.98: hepacivirin.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S96 A97 M139 K141 I160 Y162 R168 K172 F193 G283 L285 L466	S97 A98 M140 K142 I161 Y163 R169 K173 F194 G284 L286 L459
BS02	rna	A	D319 C366 S367 R386 R394 N406 S407 G410 Q414 G444	D320 C367 S368 R387 R395 N407 S408 G411 Q415 G445
BS03	MN	A	D220 D318 D319	D221 D319 D320
BS04	MN	A	D220 T221 D318	D221 T222 D319
BS05	MN	A	E237 H254	E238 H255
BS06	5GS	A	K141 R158 D220 H223 F224 D225 S282 N291 D318	K142 R159 D221 H224 F225 D226 S283 N292 D319

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4wtf, PDBe:4wtf, PDBj:4wtf
• PDBsum: 4wtf
• PubMed: 25678663
• UniProt: Q99IB8|POLG_HCVJF Genome polyprotein