Structure of PDB 4wj4 Chain A

Receptor sequence

>4wj4A (length=585) Species: 208964 (Pseudomonas aeruginosa PAO1) [Search protein sequence]

MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFD
PDRAETFAKADRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELE
VLNQAETPPFPLDEYSDVGEETRLRYRFIDLRRPEMAAKLKLRARITSSI
RRYLDDNGFLDVETPILGRPTPEGARDYLVPSRTYPGHFFALPQSPQLFK
QLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSFLDESDIIGIT
EKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVADQ
LKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKG
LAYIKVNERAKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADK
AKIVCDALGALRIKVGHDLKLLTREWAPMWVVDFPMFEENDDGSLSALHH
PFTSPKCTPAELEANPGAALSRAYDMVLNGTELGGGSIRIHDKSMQQAVF
RVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVMLMTGASSIRE
VIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLRE

3D structure

PDB

4wj4 Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis

Chain

Resolution

3.294 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R220 E222 R228 Q229 E483 G486 R538
Catalytic site (residue number reindexed from 1)	R219 E221 R227 Q228 E482 G485 R537
Enzyme Commision number	6.1.1.23: aspartate--tRNA(Asn) ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R27 R29 D30 H31 G32 G33 V34 F36 Q47 R65 R77 P80 A83 N85 E94 T108 P110 D114 E115 D118 E122 A176 E222 D223 R225 A226 R228 Y347 A448 H450 H451 R550 T558 Q559 S560	R26 R28 D29 H30 G31 G32 V33 F35 Q46 R64 R76 P79 A82 N84 E93 T107 P109 D113 E114 D117 E121 A175 E221 D222 R224 A225 R227 Y346 A447 H449 H450 R549 T557 Q558 S559
BS02	ASP	A	S196 Q198 H451 R490 L532	S195 Q197 H450 R489 L531

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004815	aspartate-tRNA ligase activity
GO:0005524	ATP binding
GO:0016874	ligase activity
GO:0050560	aspartate-tRNA(Asn) ligase activity

	Biological Process
GO:0006412	translation
GO:0006418	tRNA aminoacylation for protein translation
GO:0006422	aspartyl-tRNA aminoacylation

	Cellular Component
GO:0005737	cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:4wj4, PDBe:4wj4, PDBj:4wj4
PDBsum	4wj4
PubMed	25548166
UniProt	Q51422\|SYDND_PSEAE Aspartate--tRNA(Asp/Asn) ligase (Gene Name=aspS)

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