Structure of PDB 4weg Chain A

Receptor sequence

>4wegA (length=388) Species: 384509 (Influenza A virus (A/tern/Australia/G70C/1975(H11N9)))

RDFNNLTKGLCTINSWHIYGKDNAVRIGEDSDVLVTREPYVSCDPDECRF
YALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSS
TSCHDGKTRMSICISGPNNNASAVIWYNRRPVTEINTWARNILRTQESEC
VCHNGVCPVVFTDGSATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSC
YGERAEITCTCRDNWQGSNRPVIRIDPVAMTHTSQYICSPVLTDNPRPND
PTVGKCNDPYPGNNNNGVKGFSYLDGVNTWLGRTISIASRSGYEMLKVPN
ALTDDKSKPTQGQTIVLNTDWSGYSGSFMDYWAEGECYRACFYVELIRGR
PKEDKVWWTSNSIVSMCSSTEFLGQWDWPDGAKIEYFL

3D structure

• PDB: 4weg Catalytic mechanism and novel receptor binding sites of human parainfluenza virus type 3 hemagglutinin-neuraminidase (hPIV3 HN)
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D151 E278 R293 R371 Y405
• Catalytic site (residue number reindexed from 1): D70 E197 R212 R290 Y324
• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SFJ	A	S367 S370 S372 N399 T400 W402 K433	S286 S289 S291 N318 T319 W321 K352
BS02	CA	A	D294 G298 D325 N347	D213 G217 D244 N266
BS03	DF4	A	R118 E119 D151 R152 W179 A247 E277 E278 R293 R371 Y405	R37 E38 D70 R71 W98 A166 E196 E197 R212 R290 Y324
BS04	FSI	A	R118 E119 D151 W179 E277 E278 R293 R371 Y405	R37 E38 D70 W98 E196 E197 R212 R290 Y324

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0046761 viral budding from plasma membrane

Cellular Component

• GO:0016020 membrane
• GO:0033644 host cell membrane
• GO:0055036 virion membrane

External links

• PDB: RCSB:4weg, PDBe:4weg, PDBj:4weg
• PDBsum: 4weg
• PubMed: 26364554
• UniProt: P03472|NRAM_I75A5 Neuraminidase (Gene Name=NA)