Structure of PDB 4v0r Chain A

Receptor sequence

>4v0rA (length=852) Species: 11069 (dengue virus type 3)

GETLGEKWKKKLNQLSRKEFDLYKKSGITEVDRTEAKEGLKRGETTHHAV
SRGSAKLQWFVERNMVIPEGRVIDLGCGRGGWSYYCAGLKKVTEVRGYTK
GGPGHEEPVPMSTYGWNIVKLMSGKDVFYLPPEKCDTLLCDIGESSPSPT
VEESRTIRVLKMVEPWLKNNQFCIKVLNPYMPTVIEHLERLQRKHGGMLV
RNPLSRNSTHEMYWISNGTGNIVSSVNMVSRLLLNRFTMTHRRPTIEKDV
DLGAGTRHVNAEPETPNMDVIGERIKRIKEEHNSTWHYDDENPYKTWAYH
GSYEVKATGSASSMINGVVKLLTKPWDVVPMVTQMAMTDTTPFGQQRVFK
EKVDTRTPRPLPGTRKVMEITAEWLWRTLGRNKRPRLCTREEFTKKVRTN
YDSAKAAVEDEEFWKLVDRERELHKLGKCGSCVYNMMGSRAIWYMWLGVR
YLEFEALGFLNEDHWFSRENSYSGVEGEGLHKLGYILRDISKIPGGAMYA
DDTAGWDTRITEDDLHNEEKIIQQMDPEHRQLANAIFKLTYQNKVVKVQR
PTPTGTVMDIISRKDQRGSGQVGTYGLNTFTNMEAQLVRQMEGEGVLTKA
DLENPHLLEKKITQWLETKGVERLKRMAISGDDCVVKPIDDRFANALLAL
NDMGKVRKDIPQWQPSKGWHDWQQVPFCSHHFHELIMKDGRKLVVPCRPQ
DELIGRARISQGAGWSLRETACLGKAYAQMWSLMYFHRRDLRLASNAICS
AVPVHWVPTSRTTWSIHAHHQWMTTEDMLTVWNRVWIEENPWMEDKTPVT
TWENVPYLGKREDQWCGSLIGLTSRATWAQNIPTAIQQVRSLIGNEEFLD
YM

3D structure

• PDB: 4v0r A Crystal Structure of the Dengue Virus Ns5 Protein Reveals a Novel Inter-Domain Interface Essential for Protein Flexibility and Virus Replication.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.91: flavivirin.
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	E437 H441 C446 C449	E420 H424 C429 C432
BS02	ZN	A	H712 H714 C728 C847	H681 H683 C697 C816
BS03	SAH	A	S56 G58 G81 G86 W87 T104 K105 H110 E111 D131 V132 F133 D146	S51 G53 G76 G81 W82 T99 K100 H105 E106 D126 V127 F128 D141
BS04	GTP	A	K14 L17 N18 L20 R22 F25 S151 P152 R211 S213	K9 L12 N13 L15 R17 F20 S146 P147 R206 S208
BS05	MG	A	D533 D664	D502 D633

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
• GO:0005524 ATP binding
• GO:0008168 methyltransferase activity

Biological Process

• GO:0032259 methylation
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4v0r, PDBe:4v0r, PDBj:4v0r
• PDBsum: 4v0r
• PubMed: 25775415
• UniProt: Q5UB51|POLG_DEN3I Genome polyprotein