Structure of PDB 4uzi Chain A

Receptor sequence

>4uziA (length=446) Species: 29892 (Auricularia auricula-judae)

SLNTDDIQGDILVGMHKQKQLFYFFAINDPATFKTHLASDIAPVVASVTQ
LSNVATQPLVALNIAFSNTGLLALGVTDNLGDSLFANGQAKDATSFKEST
SSWVPQFAGTGIHGVIILASDTTDLIDQQVASIESTFGSSISKLYSLSAS
IRPGNEAGHEMFGFLDGIAQPAINGFNTPLPGQNIVDAGVIITGATNDPI
TRPSWAVGGSFLAFRQLEQLVPEFNKYLLDNAPAGSGSLQARADLLGARM
VGRWKSGAPIDLTPTADDPALGADAQRNNNFTYSHAGFDLGSDQSHCPFS
AHIRKTRPRADLGGSLTPPNLSAGANSIMRSGIPYGPEVTSAESASNTTT
QERGLAFVAYQAQLSQGFHFLQQTWADNANFPPGKTPATVGLDPIIGQNN
GQPRVVNGLLPSNSSASLSIPQFVVSHGGEYFFSPPISAIGGRLSA

3D structure

• PDB: 4uzi The Toolbox of Auricularia Auricula-Judae Dye-Decolorizing Peroxidase - Identification of Three New Potential Substrate-Interaction Sites.
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 1.11.1.19: dye decolorizing peroxidase.
  1.11.1.7: peroxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	D168 G169 I170 A171 Q221 H304 I305 T308 R309 R332 L357 F359 I397 I398 V426	D166 G167 I168 A169 Q219 H302 I303 T306 R307 R330 L355 F357 I395 I396 V424
BS02	IMD	A	D168 R332 L357 F359	D166 R330 L355 F357	MOAD: Ki=5.78mM
BS03	IMD	A	L14 V15 G16 Q172 R332	L12 V13 G14 Q170 R330	MOAD: Ki=5.78mM
BS04	EPE	A	Q108 K145 L146 S148	Q106 K143 L144 S146
BS05	OXD	A	S346 N349	S344 N347

Gene Ontology

Molecular Function

• GO:0004601 peroxidase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0140825 lactoperoxidase activity

Biological Process

• GO:0098869 cellular oxidant detoxification

Cellular Component

• GO:0005576 extracellular region
• GO:0005829 cytosol

External links

• PDB: RCSB:4uzi, PDBe:4uzi, PDBj:4uzi
• PDBsum: 4uzi
• PubMed: 25542606
• UniProt: I2DBY1|DYP_AURAJ Dye-decolorizing peroxidase AauDyP1 (Gene Name=dyp1)