Structure of PDB 4u7c Chain A

Receptor sequence

>4u7cA (length=431) Species: 9606 (Homo sapiens)

DKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQLRK
AQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPIAVG
SMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVSKEV
KEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKQNSVVFG
TSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILP
NRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLF
SETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQE
LCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIFAI
AKELLKTEIDADFPHPLRLRLMGVRISSFPN

3D structure

• PDB: 4u7c Structure and mechanism of error-free replication past the major benzo[a]pyrene adduct by human DNA polymerase kappa.
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R63 S196 E199 K321 G358 V360 T361 K468 T469 R470 A471 S472 T473	R32 S165 E168 K234 G271 V273 T274 K381 T382 R383 A384 S385 T386
BS02	dna	A	T44 S47 F49 S134 M135 A151 P153 F155 P169 S388 S391 R413 K414 S415 M416 S417 V418 E419 T421 R507	T13 S16 F18 S103 M104 A120 P122 F124 P138 S301 S304 R326 K327 S328 M329 S330 V331 E332 T334 R420
BS03	MG	A	D107 M108 D198	D76 M77 D167
BS04	0KX	A	D107 M108 A110 F111 Y112 S137 T138 Y141 R144 D198 K328	D76 M77 A79 F80 Y81 S106 T107 Y110 R113 D167 K241

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4u7c, PDBe:4u7c, PDBj:4u7c
• PDBsum: 4u7c
• PubMed: 27034468
• UniProt: Q9UBT6|POLK_HUMAN DNA polymerase kappa (Gene Name=POLK)