Structure of PDB 4u2w Chain A

Receptor sequence

>4u2wA (length=223) Species: 9913 (Bos taurus)

IVGGYTCGANTVPYQVSLNSGYHFCGGSLINSQWVVSAAHCYKSGIQVRL
GEDNINVVEGNEQFISASKSIVHPSYNSNTLNNDIMLIKLKSAASLNSRV
ASISLPTSCASAGTQCLISGWGNTKSSGTSYPDVLKCLKAPILSDSSCKS
AYPGQITSNMFCAGYLEGGKDSCQGDSGGPVVCSGKLQGIVSWGSGCAQK
NKPGVYTKVCNYVSWIKQTIASN

3D structure

• PDB: 4u2w Atomic resolution crystal structure of HV-BBI protease inhibitor from amphibian skin in complex with bovine trypsin.
• Chain: A
• Resolution: 1.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): G198 S200 G201
• Catalytic site (residue number reindexed from 1): G175 S177 G178
• Enzyme Commision number: 3.4.21.4: trypsin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	F47 H63 N102 T103 L104 Q178 D194 S195 C196 Q197 G198 S200 S215 W216 G217 S218	F24 H40 N79 T80 L81 Q155 D171 S172 C173 Q174 G175 S177 S192 W193 G194 S195
BS02	CA	A	E75 N77 V80 E85	E52 N54 V57 E62

Gene Ontology

Molecular Function

• GO:0004175 endopeptidase activity
• GO:0004252 serine-type endopeptidase activity
• GO:0005515 protein binding
• GO:0008236 serine-type peptidase activity
• GO:0046872 metal ion binding
• GO:0097655 serpin family protein binding

Biological Process

• GO:0006508 proteolysis
• GO:0007586 digestion

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0097180 serine protease inhibitor complex

External links

• PDB: RCSB:4u2w, PDBe:4u2w, PDBj:4u2w
• PDBsum: 4u2w
• PubMed: 25546528
• UniProt: P00760|TRY1_BOVIN Serine protease 1 (Gene Name=PRSS1)