Structure of PDB 4tyy Chain A

Receptor sequence

>4tyyA (length=505) Species: 559292 (Saccharomyces cerevisiae S288C)

SKLIHVPKSKEVTLDSLLEEGVLDKEIHKAITRMEFPGLTPVQQKTIKPI
LSSEDHDVIARAKTGTGKTFAFLIPIFQHLINTKFDSQYMVKAVIVAPTR
DLALQIEAEVKKIHDMNYGLKKYACVSLVGGTDFRAAMNKMNKLRPNIVI
ATPGRLIDVLEKYSNKFFRFVDYKVLDEADRLLEIGFRDDLETISGILNE
KNSKSADNIKTLLFSATLDDKVQKLANNIMNKKECLFLDTVDKNEPEAHE
RIDQSVVISEKFANSIFAAVEHIKKQIKERDSNYKAIIFAPTVKFTSFLC
SILKNEFKKDLPILEFHGKITQNKRTSLVKRFKKDESGILVCTDVGARGM
DFPNVHEVLQIGVPSELANYIHRIGRTARSGKEGSSVLFICKDELPFVRE
LEDAKNIVIAKQEKYEPSEEIKSEVLEAVTEEPEDISDIVISLISSYRSC
IKEYRFSERRILPEIASTYGVLLNDPQLKIPVSRRFLDKLGLSRSPIGKA
MFEIR

3D structure

• PDB: 4tyy Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
• Chain: A
• Resolution: 2.74 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P188 R190 G220 F224 T242 G244 R245 D248 R271 T382 V383 K384 H407 G408 R415 T433 V435 S535	P98 R100 G130 F134 T152 G154 R155 D158 R181 T292 V293 K294 H317 G318 R325 T343 V345 S445
BS02	BEF	A	T154 K158 E268 R469	T64 K68 E178 R379
BS03	CDP	A	F126 T130 Q133 G155 T156 G157 K158 T159 D441 R469 S470	F36 T40 Q43 G65 T66 G67 K68 T69 D351 R379 S380

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:4tyy, PDBe:4tyy, PDBj:4tyy
• PDBsum: 4tyy
• PubMed: 25497230
• UniProt: P15424|MS116_YEAST ATP-dependent RNA helicase MSS116, mitochondrial (Gene Name=MSS116)