Structure of PDB 4tyw Chain A

Receptor sequence

>4tywA (length=508) Species: 559292 (Saccharomyces cerevisiae S288C)

SKLIHVPKEDNSKEVTLDSLLEEGVLDKEIHKAITRMEFPGLTPVQQKTI
KPILSSEDHDVIARAKTGTGKTFAFLIPIFQHLINTKFDSQYMVKAVIVA
PTRDLALQIEAEVKKIHDMNYGLKKYACVSLVGGTDFRAAMNKMNKLRPN
IVIATPGRLIDVLEKYSNKFFRFVDYKVLDEADRLLEIGFRDDLETISGI
LNEKNSKSADNIKTLLFSATLDDKVQKLANNIMNKKECLFLDTVDKNEPE
AHERIDQSVVISEKFANSIFAAVEHIKKQIKERDSNYKAIIFAPTVKFTS
FLCSILKNEFKKDLPILEFHGKITQNKRTSLVKRFKKDESGILVCTDVGA
RGMDFPNVHEVLQIGVPSELANYIHRIGRTARSGKEGSSVLFICKDELPF
VRELEDAKNIVIAKQEKYEPSEEIKSEVLEAVTEEPEDISDIVISLISSY
RSCIKEYRFSERRILPEIASTYGVLLNDPQLKIPVSRRFLDKLGLSRSPI
GKAMFEIR

3D structure

• PDB: 4tyw Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
• Chain: A
• Resolution: 2.197 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P188 T189 R190 G220 G221 T242 G244 R245 D248 R271 F277 V383 K384 H407 G408 R415 T433 S532 S535	P101 T102 R103 G133 G134 T155 G157 R158 D161 R184 F190 V296 K297 H320 G321 R328 T346 S445 S448
BS02	MG	A	F442 V445 R466 R469	F355 V358 R379 R382
BS03	BEF	A	T154 K158 R466 R469	T67 K71 R379 R382
BS04	ADP	A	F126 T130 Q133 G155 G157 K158 T159 D441 R469 S470	F39 T43 Q46 G68 G70 K71 T72 D354 R382 S383

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:4tyw, PDBe:4tyw, PDBj:4tyw
• PDBsum: 4tyw
• PubMed: 25497230
• UniProt: P15424|MS116_YEAST ATP-dependent RNA helicase MSS116, mitochondrial (Gene Name=MSS116)