Structure of PDB 4tyn Chain A

Receptor sequence

>4tynA (length=509) Species: 559292 (Saccharomyces cerevisiae S288C)

SKLIHVPKEDNSKEVTLDSLLEEGVLDKEIHKAITRMEFPGLTPVQQKTI
KPILSSEDHDVIARAKTGTGKTFAFLIPIFQHLINTKFDSQYMVKAVIVA
PTRDLALQIEAEVKKIHDMNYGLKKYACVSLVGGTDFRAAMNKMNKLRPN
IVIATPGRLIDVLEKYSNKFFRFVDYKVLDEADRLLEIGFRDDLETISGI
LNEKNSKSADNIKTLLFSATLDDKVQKLANNIMNKKECLFLDTVDKNEPE
AHERIDQSVVISEKFANSIFAAVEHIKKQIKERDSNYKAIIFAPTVKFTS
FLCSILKNEFKKDLPILEFHGKITQNKRTSLVKRFKKDESGILVCTDVGA
RGMDFPNVHEVLQIGVPSELANYIHRIGRTARSGKEGSSVLFICKDELPF
VRELEDAKNIVIAKQEKYEPSEEIKSEVLEAVTEEPEDISDIVISLISSY
RSCIKEYRFSERRILPEIASTYGVLLNDPQLKIPVSRRFLDKLGLSRSPI
GKAMFEIRD

3D structure

• PDB: 4tyn Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase.
• Chain: A
• Resolution: 2.959 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R190 G220 T242 G244 D248 R271 P381 T382 G408 R415 T433 S455 E456 S532 S535 S539	R103 G133 T155 G157 D161 R184 P294 T295 G321 R328 T346 S368 E369 S445 S448 S452
BS02	MG	A	T154 K158	T67 K71
BS03	BEF	A	D267 E268 G439	D180 E181 G352
BS04	ADP	A	F126 T130 Q133 G155 T156 G157 K158 T159 D441 R469 S470	F39 T43 Q46 G68 T69 G70 K71 T72 D354 R382 S383

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:4tyn, PDBe:4tyn, PDBj:4tyn
• PDBsum: 4tyn
• PubMed: 25497230
• UniProt: P15424|MS116_YEAST ATP-dependent RNA helicase MSS116, mitochondrial (Gene Name=MSS116)