Structure of PDB 4ty8 Chain A

Receptor sequence

>4ty8A (length=544)

SMSYTWTGALITPCAAEESKLPNSLLRHHNMVYATTSRSAGLRQKKVTFD
RLQVLDDHYRDVLKEMKAKASTVKAKLLSVEEACKLTPPHSAKSKFGYGA
KDVRNLSSKAVNHIHSVWKDLLEDTVTPIDTTIMAKNEVFCVQRKPARLI
VFPDLGVRVCEKMALYDVVSTLPQVVMGSSYGFQYSPGQRVEFLVNTWKS
KKNPMGFSYDTRCFDSTVTENDIRVEESIYQCCDLAPEARQAIKSLTERL
YIGGPLTNSKGQNCGYRRCRASGVLTTSCGNTLTCYLKASAACRAAKLQD
CTMLVNGDDLVVICESAGTQEDAASLRVFTEAMTRYSAPPGDPPQPEYDL
ELITSCSSNVSVAHDASGKRVYYLTRDPTTPLARAAWETARHTPVNSWLG
NIIMYAPTLWARMILMTHFFSILLAQEQLEKALDCQIYGACYSIEPLDLP
QIIERLHGLSAFSLHSYSPGEINRVASCLRKLGVPPLRVWRHRARSVRAR
LLSQGGRAATCGKYLFNWAVKTKLKLTPISGWFVAGYSGGDIYH

3D structure

• PDB: 4ty8 A ligand-observed mass spectrometry approach integrated into the fragment based lead discovery pipeline
• Chain: A
• Resolution: 2.78 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	3AV	A	P197 C366 S368 Y448	P187 C356 S358 Y438	PDBbind-CN: -logKd/Ki=2.03,Kd=9.31mM

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0008236 serine-type peptidase activity
• GO:0016887 ATP hydrolysis activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0039654 fusion of virus membrane with host endosome membrane
• GO:0039694 viral RNA genome replication
• GO:0046718 symbiont entry into host cell

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:4ty8, PDBe:4ty8, PDBj:4ty8
• PDBsum: 4ty8
• PubMed: 25666181
• UniProt: D0PY27