Structure of PDB 4tmu Chain A

Receptor sequence

>4tmuA (length=511) Species: 290339 (Cronobacter sakazakii ATCC BAA-894)

AEVYSQETLAKQVLQETFGYQQFRPGQATIIDAVLEGRDCLVVMPTGGGK
SLCYQIPALVKTGLTIVVSPLISLMKDQVDQLLANGVAAACLNSTQSREE
QQAVLAGCRTGQVRLLYIAPERLMMDNFIDTLGYWDLAMVAVDEAHCISQ
WGHDFRPEYAALGQLRARFPAVPFMALTATADDTTRRDIVRLLGLDDPLI
EISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTA
ARLQSRGISAAAYHAGLEHEVRASVQEKFQRDDLQIVVATVAFGMGINKP
NVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLE
EKAPGPLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQAPCGNCDIC
LDPPRRYDGLVDAQKALSAIARVEQRFGMGYVVEVLRGANNQRIRELGHD
KLKVYGIGRDQSQEHWVSVIRQLIHLGVVTQNIAQHSALQLTEAARPFLR
GEAPLMLAVPR

3D structure

• PDB: 4tmu Structural mechanisms of DNA binding and unwinding in bacterial RecQ helicases.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L74 I75 N96 S97 E124 R125 W154 F158 R159 P223 L224 S245 R246 R259 H267 A268 T293 V294 R315 W347 M432 G433 A442 N443 N444 R446 H489 S490	L71 I72 N93 S94 E121 R122 W151 F155 R156 P220 L221 S242 R243 R256 H264 A265 T290 V291 R312 W344 M429 G430 A439 N440 N441 R443 H486 S487	PDBbind-CN: Kd=1.5nM
BS02	ZN	A	C380 C397 C400 C403	C377 C394 C397 C400

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0043138 3'-5' DNA helicase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0009432 SOS response

External links

• PDB: RCSB:4tmu, PDBe:4tmu, PDBj:4tmu
• PDBsum: 4tmu
• PubMed: 25831501
• UniProt: A7MQK9