Structure of PDB 4tmk Chain A

Receptor sequence
>4tmkA (length=210) Species: 562 (Escherichia coli) [Search protein sequence]
RSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGTQLAEKLR
SLLLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRH
DLSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRAR
ARGELDRIEQESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIR
TTVTHWVKEL
3D structure
PDB4tmk Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase.
ChainA
Resolution1.98 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.4.9: dTMP kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 T5A A E12 G13 G15 K16 T17 R39 E40 L55 F74 R78 R100 S104 T105 Y108 Q109 R149 R153 I159 E11 G12 G14 K15 T16 R38 E39 L54 F73 R77 R99 S103 T104 Y107 Q108 R148 R152 I158 MOAD: Kd=20nM
PDBbind-CN: -logKd/Ki=7.70,Kd=20nM
Gene Ontology
Molecular Function
GO:0004798 thymidylate kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0042803 protein homodimerization activity
Biological Process
GO:0006227 dUDP biosynthetic process
GO:0006233 dTDP biosynthetic process
GO:0006235 dTTP biosynthetic process
GO:0009165 nucleotide biosynthetic process
GO:0015949 nucleobase-containing small molecule interconversion
GO:0016310 phosphorylation
GO:0046940 nucleoside monophosphate phosphorylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:4tmk, PDBe:4tmk, PDBj:4tmk
PDBsum4tmk
PubMed9826650
UniProtP0A720|KTHY_ECOLI Thymidylate kinase (Gene Name=tmk)

[Back to BioLiP]