Structure of PDB 4tf4 Chain A

Receptor sequence

>4tf4A (length=605) Species: 2021 (Thermobifida fusca)

EPAFNYAEALQKSMFFYEAQRSGKLPENNRVSWRGDSGLNDGADVGLDLT
GGWYDAGDHVKFGFPMAFTATMLAWGAIESPEGYIRSGQMPYLKDNLRWV
NDYFIKAHPSPNVLYVQVGDGDADHKWWGPAEVMPMERPSFKVDPSCPGS
DVAAETAAAMAASSIVFADDDPAYAATLVQHAKQLYTFADTYRGVYSDCV
PAGAFYNSWSGYQDELVWGAYWLYKATGDDSYLAKAEYEYDFLSTEQQTD
LRSYRWTIAWDDKSYGTYVLLAKETGKQKYIDDANRWLDYWTVGVNGQRV
PYSPGGMAVLDTWGALRYAANTAFVALVYAKVIDDPVRKQRYHDFAVRQI
NYALGDNPRNSSYVVGFGNNPPRNPHHRTAHGSWTDSIASPAENRHVLYG
ALVGGPGSPNDAYTDDRQDYVANEVATDYNAGFSSALAMLVEEYGGTPLA
DFPPTEEPDGPEIFVEAQINTPGTTFTEIKAMIRNQSGWPARMLDKGTFR
YWFTLDEGVDPADITVSSAYNQCATPEDVHHVSGDLYYVEIDCTGEKIFP
GGQSEHRREVQFRIAGGPGWDPSNDWSFQGIGNELAPAPYIVLYDDGVPV
WGTAP

3D structure

• PDB: 4tf4 Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D55 D58 Y206 E424
• Catalytic site (residue number reindexed from 1): D55 D58 Y206 E424
• Enzyme Commision number: 3.2.1.4: cellulase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	D58 F205 Y206 W313 E424	D58 F205 Y206 W313 E424
BS02	BGC	A	F205 R317	F205 R317
BS03	BGC	A	W256 D261 D262	W256 D261 D262
BS04	BGC	A	H376 R378 Y420 E424	H376 R378 Y420 E424
BS05	CA	A	S210 G211 D214 E215 D261	S210 G211 D214 E215 D261
BS06	CA	A	T504 D506 D571 N574 D575	T504 D506 D571 N574 D575

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0030246 carbohydrate binding
• GO:0030248 cellulose binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:4tf4, PDBe:4tf4, PDBj:4tf4
• PDBsum: 4tf4
• PubMed: 9334746
• UniProt: P26221|GUN4_THEFU Endoglucanase E-4 (Gene Name=celD)