Structure of PDB 4rve Chain A

Receptor sequence
>4rveA (length=240) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTY
NINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIY
3D structure
PDB4rve The crystal structure of EcoRV endonuclease and of its complexes with cognate and non-cognate DNA fragments.
ChainA
Resolution3.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A K38 Q68 Q69 N70 S183 N185 S223 K37 Q67 Q68 N69 S182 N184 S222
BS02 dna A T37 S41 N70 K92 T93 T94 Y95 K119 N120 G182 S183 T186 T36 S40 N69 K91 T92 T93 Y94 K118 N119 G181 S182 T185
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:4rve, PDBe:4rve, PDBj:4rve
PDBsum4rve
PubMed8491171
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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