Structure of PDB 4ruc Chain A

Receptor sequence

>4rucA (length=341) Species: 273057 (Saccharolobus solfataricus P2) [Search protein sequence]

MIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEA
RKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIE
IASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVF
AKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGIN
KLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIVT
MKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGR
TFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFI

3D structure

PDB

4ruc Structural Basis for Error-Free Bypass of the 5-N-Methylformamidopyrimidine-dG Lesion by Human DNA Polymerase eta and Sulfolobus solfataricus P2 Polymerase IV.

Chain

Resolution

2.9 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	V32 F37 S40 G41 A42 P60 G218 E219 A220 R242 K243 S244 G246 R247 I248 L293 R331 R332 R336	V32 F37 S40 G41 A42 P60 G218 E219 A220 R242 K243 S244 G246 R247 I248 L293 R331 R332 R336
BS02	dna	A	S103 E106 G185 I186 G187 I189 T190 S297 R298 R300 T301	S103 E106 G185 I186 G187 I189 T190 S297 R298 R300 T301
BS03	DTP	A	D7 F8 Y10 F11 Y12 A44 T45 R51 D105 K159	D7 F8 Y10 F11 Y12 A44 T45 R51 D105 K159
BS04	CA	A	D7 D105 E106	D7 D105 E106
BS05	CA	A	D7 F8 D105	D7 F8 D105
BS06	CA	A	A181 I186	A181 I186

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003677	DNA binding
GO:0003684	damaged DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006281	DNA repair
GO:0006974	DNA damage response
GO:0042276	error-prone translesion synthesis

	Cellular Component
GO:0005737	cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:4ruc, PDBe:4ruc, PDBj:4ruc
PDBsum	4ruc
PubMed	25988947
UniProt	Q97W02\|DPO4_SACS2 DNA polymerase IV (Gene Name=dbh)

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