Structure of PDB 4ru9 Chain A

Receptor sequence

>4ru9A (length=427) Species: 9606 (Homo sapiens)

MATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAV
SYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVM
EIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGL
PQGPTVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIE
RETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKI
RSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGI
EHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKD
RNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKN
CNTSGIQTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 4ru9 Structural Basis for Error-Free Bypass of the 5-N-Methylformamidopyrimidine-dG Lesion by Human DNA Polymerase eta and Sulfolobus solfataricus P2 Polymerase IV.
• Chain: A
• Resolution: 2.65 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W42 G46 I47 S62 W64 K86 K293 K311 R313 P316 K317 T318 G320 S322 K323 N324 P326 R351	Q38 Y39 W42 G46 I47 S62 W64 K86 K288 K306 R308 P311 K312 T313 G315 S317 K318 N319 P321 R346
BS02	dna	A	S113 E116 K224 S257 G259 G260 K261 L262 R382 R383	S113 E116 K219 S252 G254 G255 K256 L257 R377 R378
BS03	0KX	A	D13 M14 D15 C16 F17 F18 Y52 R55 R61 D115 K231	D13 M14 D15 C16 F17 F18 Y52 R55 R61 D115 K226
BS04	MG	A	D13 M14 D115	D13 M14 D115
BS05	MG	A	D13 D115 E116	D13 D115 E116

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4ru9, PDBe:4ru9, PDBj:4ru9
• PDBsum: 4ru9
• PubMed: 25988947
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)