Structure of PDB 4rsc Chain A

Receptor sequence
>4rscA (length=504) Species: 9913 (Bos taurus) [Search protein sequence]
SQVEHPAGGYKKLFETVEELSSPLTAHVTGRIPLWLTGSLLRCGPGLFEV
GSEPFYHLFDGQALLHKFDFKEGHVTYHRRFIRTDAYVRAMTEKRIVITE
FGTCAFEVTDNALVNIYPVGEDYYACTETNFITKVNPETLETIKQVDLCN
YVSVNGATAHPHIENDGTVYNIGNCFIAYNIVKIPPLQADKEDPISKSEI
VVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSGANYMDCF
ESNETMGVWLHIADKKRKKYINNKYRTSPFNLFHHINTYEDHEFLIVDLC
CWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADT
GKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYGGK
PYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDAL
EEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGL
FKKS
3D structure
PDB4rsc Catalytic mechanism of a retinoid isomerase essential for vertebrate vision.
ChainA
Resolution1.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.1.64: retinoid isomerohydrolase.
5.3.3.22: lutein isomerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FE2 A H180 H241 H313 H527 H160 H216 H284 H498
BS02 A3V A F61 F103 V134 T147 E148 N194 F59 F101 V114 T127 E128 N174 MOAD: ic50=232nM
PDBbind-CN: -logKd/Ki=6.63,IC50=232nM
BindingDB: IC50=<1000nM
Gene Ontology
Molecular Function
GO:0001786 phosphatidylserine binding
GO:0003834 beta-carotene 15,15'-dioxygenase activity
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0031210 phosphatidylcholine binding
GO:0042802 identical protein binding
GO:0046872 metal ion binding
GO:0050251 retinol isomerase activity
GO:0052884 all-trans-retinyl-palmitate hydrolase, 11-cis retinol forming activity
GO:0052885 all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity
GO:1901612 cardiolipin binding
Biological Process
GO:0006629 lipid metabolic process
GO:0007601 visual perception
GO:0042572 retinol metabolic process
GO:0042574 retinal metabolic process
GO:1901827 zeaxanthin biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005886 plasma membrane
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4rsc, PDBe:4rsc, PDBj:4rsc
PDBsum4rsc
PubMed25894083
UniProtQ28175|RPE65_BOVIN Retinoid isomerohydrolase (Gene Name=RPE65)

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