Structure of PDB 4rib Chain A

Receptor sequence

>4ribA (length=615) Species: 9606 (Homo sapiens)

TGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQLSATGQKLYV
RLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTESELQELSEVL
ELLSAPELKSLAKTFHLANPNGQKQQLVDAFLKLAKQRSVIGAVILKRAK
ALAGQSVRICKGPRAVFSRILLLFSLTDSMEDEDAACGGQGQLSTVLLVN
LGRMEFPSYTINRKTHIFQDRDDLIRYAAATHMLSDISSAMANGNWEEAK
ELAQCAKRDWNRLKNHPSLRCHEDLPLFLRCFTVGWIYTRILSRFVEILQ
RLHMYEEAVRELESLLSQRIYCPDSRGRWWDRLALNLHQHLKRLEPTIKC
ITEGLADPEVRTGHRLSLYQRAVRLRESPSCKKFKHLFQQLPEMAVQDVK
HVTITGRLCKSVFVMVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGL
LLWDIIFMDGIPDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAP
EESLRAWVAATWHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVC
RHLAADFRHCRGGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAEL
QKLGAEVEVCHVVAV

3D structure

• PDB: 4rib DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1.
• Chain: A
• Resolution: 3.25 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.-
  3.1.4.1: phosphodiesterase I.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Y374 R420 R424 K425 Y436 T573	Y5 R51 R55 K56 Y67 T195	PDBbind-CN: Kd=10.3nM
BS02	dna	A	E675 R706 R710 P757 R955 G956 G957 D960 N980 D981	E297 R328 R332 P379 R561 G562 G563 D566 N586 D587	PDBbind-CN: Kd=10.3nM
BS03	dna	A	K433 S473 Q492 K493 T573	K64 S104 Q123 K124 T195	PDBbind-CN: Kd=10.3nM
BS04	dna	A	R679 H681 R710 Q717 H718 R749 R752 R982	R301 H303 R332 Q339 H340 R371 R374 R588	PDBbind-CN: Kd=10.3nM
BS05	CA	A	D960 E975 V976	D566 E581 V582

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004518 nuclease activity
• GO:0016788 hydrolase activity, acting on ester bonds

Biological Process

• GO:0036297 interstrand cross-link repair

External links

• PDB: RCSB:4rib, PDBe:4rib, PDBj:4rib
• PDBsum: 4rib
• PubMed: 25430771
• UniProt: Q9Y2M0|FAN1_HUMAN Fanconi-associated nuclease 1 (Gene Name=FAN1)