Structure of PDB 4ri8 Chain A

Receptor sequence

>4ri8A (length=615) Species: 9606 (Homo sapiens)

TGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQLSATGQKLYV
RLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTESELQELSEVL
ELLSAPELKSLAKTFHLANPNGQKQQLVDAFLKLAKQRSVIGAVILKRAK
ALAGQSVRICKGPRAVFSRILLLFSLTDSMEDEDAACGGQGQLSTVLLVN
LGRMEFPSYTINRKTHIFQDRDDLIRYAAATHMLSDISSAMANGNWEEAK
ELAQCAKRDWNRLKNHPSLRCHEDLPLFLRCFTVGWIYTRILSRFVEILQ
RLHMYEEAVRELESLLSQRIYCPDSRGRWWDRLALNLHQHLKRLEPTIKC
ITEGLADPEVRTGHRLSLYQRAVRLRESPSCKKFKHLFQQLPEMAVQDVK
HVTITGRLCKSVFVMVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGL
LLWDIIFMDGIPDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAP
EESLRAWVAATWHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVC
RHLAADFRHCRGGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAEL
QKLGAEVEVCHVVAV

3D structure

• PDB: 4ri8 DNA repair. Mechanism of DNA interstrand cross-link processing by repair nuclease FAN1.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.-
  3.1.4.1: phosphodiesterase I.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	P373 Y374 R420 R424 K425 Y436 T573 V577 R581	P4 Y5 R51 R55 K56 Y67 T195 V199 R203
BS02	dna	A	R706 H742 K794 S795 R952 R955 G956 G957 N980 K986	R328 H364 K410 S411 R558 R561 G562 G563 N586 K592
BS03	dna	A	K433 S473 A474 Q492 K493 R679 R710 L713 H718 R749 R752	K64 S104 A105 Q123 K124 R301 R332 L335 H340 R371 R374
BS04	CA	A	D960 E975 V976	D566 E581 V582

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004518 nuclease activity
• GO:0016788 hydrolase activity, acting on ester bonds

Biological Process

• GO:0036297 interstrand cross-link repair

External links

• PDB: RCSB:4ri8, PDBe:4ri8, PDBj:4ri8
• PDBsum: 4ri8
• PubMed: 25430771
• UniProt: Q9Y2M0|FAN1_HUMAN Fanconi-associated nuclease 1 (Gene Name=FAN1)