Structure of PDB 4rh7 Chain A

Receptor sequence

>4rh7A (length=3005) Species: 32630 (synthetic construct)

QGEVTIREALRELDLWGVGAVFTLIDYEDSQSRTMKLIKDWKDIVNQVGD
NRCLLQSLKDSPYYKGFEDKVSIWERKLAELDEYLQNLNHIQRKWVYLEP
IFGRGALPKEQTRFNRVDEDFRSIMTDIKKDNRVTTLTTHAGIRNSLLTI
LDQLQRCQRSLNEFLEEKRSAFPRFYFIGDDDLLEILGQSTNPSVIQSHL
KKLFAGINSVCFDEKSKHITAMKSLEGEVVPFKNKVPLSNNVETWLNDLA
LEMKKTLEQLLKECVTTGRSSQGAVDPSLFPSQILCLAEQIKFTEDVENA
IKDHSLHQIETQLVNKLEQYTNIDTSSEDPGNTESGILELKLKALILDII
HNIDVVKQLNQIQVHTTEDWAWKKQLRFYMKSDHTCCVQMVDSEFQYTYE
YQGNASKLVYTPLTDKCYLTLTQAMKMGLGGNPYGPAGTGKTESVKALGG
LLGRQVLVFNCDEGIDVKSMGRIFVGLVKCGAWGCFDEFNRLEESVLSAV
SMQIQTIQDALKNHRTVCELLGKEVEVNSNSGIFITMNPAGKGYGGRQKL
PDNLKQLFRPVAMSHPDNELIAEVILYSEGFKDAKVLSRKLVAIFNLSRE
LLTPQQHYDWGLRALKTVLRGSGNLLRQLNKSGTTQNANESHIVVQALRL
NTMSKFTFTDCTRFDALIKDVFPGIELKEVEYDELSAALKQVFEEANYEI
IPNQIKKALELYEQLCQRMGVVIVGPSGAGKSTLWRMLRAALCKTGKVVK
QYTMNPKAMPRYQLLGHIDMDTREWSDGVLTNSARQVVREPQDVSSWIIC
DGDIDPEWIESLNSVLDDNRLLTMPSGERIQFGPNVNFVFETHDLSCASP
ATISRMGMIFLSDEETDLNSLIKSWLRNQPAEYRNNLENWIGDYFEKALQ
WVLKQNDYVVETSLVGTVMNGLSHLHGCRDHDEFIINLIRGLGGNLNMKS
RLEFTKEVFHWARESPPDFHKPMDTYYDSTRGRLATYVLKKPEDLTADDF
SNGLTLPVIQTPDMQRGLDYFKPWLSSDTKQPFILVGPEGCGKGMLLRYA
FSQLRSTQIATVHCSAQTTSRHLLQKLSQTCMVISTNTGRVYRPKDCERL
VLYLKDINLPKLDKWGTSTLVAFLQQVLTYQGFYDENLEWVGLENIQIVA
SMSAGGRLGRHKLTTRFTSIVRLCSIDYPEREQLQTIYGAYLEPVLHKNL
KNHSIWGSSSKIYLLAGSMVQVYEQVRAKFTVDDYSHYFFTPCILTQWVL
GLFRYDLEGGSSNHPLDYVLEIVAYEARRLFRDKIVGAKELHLFDIILTS
VFQGDWGSDILDNMSDSFYVTWGARHNSGAPGQPLPPHGKPLGKLNSTDL
KDVIKKGLIHYGRDNQNLDILLFHEVLEYMSRIDRVLSFPGGSLLLAGRS
GVGRRTITSLVSHMHGAVLFSPKISRGYELKQFKNDLKHVLQLAGIEAQQ
VVLLLEDYQFVHPTFLEMINSLLSSGEVPGLYTLEELEPLLLPLKDQASQ
DGFFGPVFNYFTYRIQQNLHIVLIMDSANSNFMINCESNPALHKKCQVLW
MEGWSNSSMKKIPEMLFSEVDPDFLKSFLLIHESCKAYGATPSQYMTFLH
VYSAISSSKKKELLKRQSHLQAGVSKLNEAKALVDELNRKAGEQSVLLKT
KQDEADAALQMITVSMQDASEQKTELERLKHRIAEEVVKIEERKNKIDDE
LKEVQPLVNEAKLAVGNIKPESLSEIRSLRMPPDVIRDILEGVLRLMGIF
DTSWVSMKSFLAKRGVREDIATFDARNISKEIRESVEELLFKNKGSFDPK
NAKRASTAAAPLAAWVKANIQYSHVLERIHPLETEQAGLESNLKKTEDRK
RKLEELLNSVGQKVSELKEKFQSRTSEAAKLEAEVSKAQETIKAAEVLIN
QLDREHKRWNAQVVEITEELATLPKRAQLAAAFITYLSAAPESLRKTCLE
EWTKSAGLEKFDLRRFLCTESEQLIWKSEGLPSDDLSIENALVILQSRVC
PFLIDPSSQATEWLKTHLKDSRLEVINQQDSNFITALELAVRFGKTLIIQ
EMDGVEPVLYPLLRRDLVAQGPRYVVQIGDKIIDYNEEFRLFLSTRNPNP
FIPPDAASIVTEVNFTTTRSGLRGQLLALTIQHEKPDLEEQKTKLLQQEE
DKKIQLAKLEESLLETLATSQGNILENKDLIESLNQTKASSALIQESLKE
SYKLQISLDQERDAYLPLAESASKMYFIISDLSKINNMYRFSLAAFLRLF
QRALQNKQDSENTEQRIQSLISSLQHMVYEYICRCLFKADQLMFALHFVR
GMHPELFQENEWDTFTGVDQLPSWIDQERSWAVATLKIALPSLYQTLCFE
DAALWRTYYNNSMCEQEFPSILAKKVSLFQQILVVQVLRPDRLQSAMALF
ACKTLGLKEVSPLPLNLKRLYKETLEIEPILIIISPGADPSQELQELANA
ERSGECYHQVAMGQGQADLAIQMLKECARNGDWLCLKNLHLVVSWLPVLE
KELNTLQPKDTFRLWLTAEVHPNFTPILLQSSLKITYESPPGLKKNLMRT
YESWTPEQISKKDNTHRAHALFSLAWFHAACQERRNYIPQGWTKFYEFSL
SDLRAGYNIIDRLFDGAKDVQWEFVHGLLENAIYGGRIDNYFDLRVLQSY
LKQFFNSSVIIFPYSVSLPQSCSILDYRAVIEKIPEDDKPSFFGLPANIA
RSSQRMISSQVISQLRILGRSITAGSKFDREIWSNELSPVLNLWKKLNQN
SNLIHQKVPPPNDRQGSPILSFIILEQFNAIRLVQSVHQSLAALSKVIRG
TTLLSSEVQKLASALLNQKCPLAWQSKWEGPEDPLQYLRGLVARALAIQN
WVDKAEKQALLSETLDLSELFHPDTFLNALRQETARAVGRSVDSLKFVAS
WKGRLQEAKLQIKISGLLLEGCSFDGNQLSENQLDSPSVSSVLPCFMGWI
PQDACGPYSPDECISLPVYTSAERDRVVTNIDVPCGGNQDQWIQCGAALF
LKNQV

3D structure

• PDB: 4rh7 Structure of human cytoplasmic dynein-2 primed for its power stroke.
• Chain: A
• Resolution: 3.41 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AOV	A	L1662 V1663 A1691 T1693 G1694 K1695 T1696 E1697 D1741 E1742 N1792 I1825 L1866 R1867 K1870 D2071 D2072 R2109	L408 V409 A437 T439 G440 K441 T442 E443 D487 E488 N538 I571 L612 R613 K616 D817 D818 R855
BS02	MG	A	T1696 D1741 R2109	T442 D487 R855
BS03	ATP	A	F1947 Y1952 E1953 Q1958 S1981 G1982 G1984 K1985 S1986 T1987 E2095 L2125 R2420	F693 Y698 E699 Q704 S727 G728 G730 K731 S732 T733 E841 L871 R1166
BS04	MG	A	S1986 E2095	S732 E841
BS05	ADP	A	V2262 I2263 G2294 G2296 K2297 G2298 M2299 Y2445 P2496 C2497 T2500	V1008 I1009 G1040 G1042 K1043 G1044 M1045 Y1191 P1242 C1243 T1246
BS06	ADP	A	I2627 L2628 G2660 R2661 R2662 T2663 M2873	I1370 L1371 G1403 R1404 R1405 T1406 M1596

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0006091 generation of precursor metabolites and energy
• GO:0007018 microtubule-based movement
• GO:0008218 bioluminescence

Cellular Component

• GO:0030286 dynein complex

External links

• PDB: RCSB:4rh7, PDBe:4rh7, PDBj:4rh7
• PDBsum: 4rh7
• PubMed: 25470043
• UniProt: Q8NCM8|DYHC2_HUMAN Cytoplasmic dynein 2 heavy chain 1 (Gene Name=DYNC2H1)