Structure of PDB 4re4 Chain A

Receptor sequence

>4re4A (length=483) Species: 39946 (Oryza sativa Indica Group)

AAPFTYWLNPEIYDAGGLSRRAFPEGFVFGTAASAYQVEGMAKQGGRGPS
IWDAFIEKPGTIPNNATADVTVDEYHRYKEDVNIMKNMGFDAYRFSISWS
RIFPNGTGMVNQEGVDYYNRLIDYMVKKGIKPYANLYHYDLPLALHEQYL
GWLSPNIVEAFADYADFCFQTFGDRVKDWFTFNEPRCVAALGYDNGFHAP
GRCSGCDAGGNSTTEPYLAAHHLILSHAAAVKRYREKYQLYQKGRIGILL
DFVWYEPFSDSNADRAAAQRARDFHLGWFLDPIIHGRYPYSMLEIVKDRM
PTFSDEESRMVKDSIDYVGINHYTSFYMKDPGPWNLTPTSYQDDWHVGFA
YERNGVPIGAQANSYWLYIVPWGINKAVTYVKETYGNPTMILSENGMDQP
GNVSITQGVHDTVRIRYYRNYITELKKAIDDGAKVIGYFAWSLLDNFEWR
LGYTSRFGIVYVDYKTLKRYPKDSAFWFKNMLS

3D structure

• PDB: 4re4 A Single Glycosidase Harnesses Different Pyranoside Ring Transition State Conformations for Hydrolysis of Mannosides and Glucosides
• Chain: A
• Resolution: 2.29 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R89 H133 E179 C182 N316 Y318 E389
• Catalytic site (residue number reindexed from 1): R94 H138 E184 C187 N321 Y323 E394
• Enzyme Commision number: 3.2.1.25: beta-mannosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	IFM	A	Q32 H133 E179 Y318 E389 W436 E443 W444 F452	Q37 H138 E184 Y323 E394 W441 E448 W449 F457	PDBbind-CN: -logKd/Ki=6.87,Ki=135nM

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0004565 beta-galactosidase activity
• GO:0008422 beta-glucosidase activity
• GO:0033907 beta-D-fucosidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:4re4, PDBe:4re4, PDBj:4re4
• PDBsum: 4re4
• UniProt: A2YPH1