Structure of PDB 4rdw Chain A

Receptor sequence

>4rdwA (length=451) Species: 208964 (Pseudomonas aeruginosa PAO1)

SAIFAERALLPEGWARNVRFEISADGVLAEIRPDANADGAERLGGAVLPG
MPNLHSHAFQRAMAGLAEVAGNPNDSFWTWRELMYRMVARLSPEQIEVIA
CQLYIEMLKAGYTAVAEFHYVHHDLDGRSYADPAELSLRISRAASAAGIG
LTLLPVLYSHAGFGGQPASEGQRRFINGSEAYLELLQRLRAPLEAAGHSL
GLCFHSLRAVTPQQIATVLAAGHDDLPVHIHIAEQQKEVDDCQAWSGRRP
LQWLYENVAVDQRWCLVHATHADPAEVAAMARSGAVAGLCLSTEANLGDG
IFPATDFLAQGGRLGIGSDSHVSLSVVEELRWLEYGQRLRDRKRNRLYRD
DQPMIGRTLYDAALAGGAQALGQPIGSLAVGRRADLLVLDGNDPYLASAE
GDALLNRWLFAGGDRQVRDVMVAGRWVVRDGRHAGEERSARAFVQVLGEL
L

3D structure

• PDB: 4rdw The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-Guanidino-L-Glutaric acid
• Chain: A
• Resolution: 1.591 Å

Enzymatic activity

• Enzyme Commision number: 3.5.3.13: formimidoylglutamate deiminase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	H56 H58 H232 D320	H55 H57 H231 D319
BS02	NGQ	A	H58 F78 R82 Y121 H206 R209 E235	H57 F77 R81 Y120 H205 R208 E234

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity
• GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
• GO:0019239 deaminase activity
• GO:0046872 metal ion binding
• GO:0050416 formimidoylglutamate deiminase activity

Biological Process

• GO:0006547 L-histidine metabolic process
• GO:0006548 L-histidine catabolic process
• GO:0019556 L-histidine catabolic process to glutamate and formamide
• GO:0019557 L-histidine catabolic process to glutamate and formate

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:4rdw, PDBe:4rdw, PDBj:4rdw
• PDBsum: 4rdw
• UniProt: Q9HU77|HUTF_PSEAE Formimidoylglutamate deiminase (Gene Name=hutF)