Structure of PDB 4rcg Chain A

Receptor sequence
>4rcgA (length=592) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]
GLDTAPTNHQGLLSWVEEVAELTQPDRVVFTDGSEEEFQRLCDQLVEAGT
FIRLNPEKHKNSYLALSDPSDVARVESRTYICSAKEIDAGPTNNWMDPGE
MRSIMKDLYRGCMRGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMR
TMTRMGKAALEKMGDDGFFVKALHSVGAPLEPGQKDVAWPCSETKYITHF
PETREIWSYGSGYGGNALLGKKCYSLRIASAMAHDEGWLAEHMLILKLIS
PENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGR
LYAVNPEFGFFGVAPGTNWKSNPNAMRTIAAGNTVFTNVALTDDGDVWWE
GLEGDPQHLIDWKGNDWYFRETETNAAHPNSRYCTPMSQCPILAPEWDDP
QGVPISGILFGGRRKTTVPLVTEARDWQHGVFIGATLGSEQTANVRRDPM
AMLPFLGYNVGDYFQHWINLGKHADEAKLPKVFFVNWFRRGDDGRFLWPG
FGENSRVLKWIVDRIEHKAGGATTPIGTVPAVEDLDLDGLDVDAADVAAA
LAVDADEWRQELPLIEEWLQFVGEKLPTGVKDEFDALKERLG
3D structure
PDB4rcg Structural and Functional Studies of Phosphoenolpyruvate Carboxykinase from Mycobacterium tuberculosis.
ChainA
Resolution2.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R81 Y220 K229 H249 S271 C273 D296 R389
Catalytic site (residue number reindexed from 1) R74 Y213 K222 H242 S264 C266 D289 R382
Enzyme Commision number 4.1.1.32: phosphoenolpyruvate carboxykinase (GTP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GDP A S271 A272 G274 K275 T276 N277 W501 F502 G514 F515 S264 A265 G267 K268 T269 N270 W487 F488 G500 F501 PDBbind-CN: -logKd/Ki=5.03,Kd=9.3uM
Gene Ontology
Molecular Function
GO:0004611 phosphoenolpyruvate carboxykinase activity
GO:0004613 phosphoenolpyruvate carboxykinase (GTP) activity
GO:0005525 GTP binding
GO:0016831 carboxy-lyase activity
GO:0017076 purine nucleotide binding
GO:0030145 manganese ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006094 gluconeogenesis
GO:0006107 oxaloacetate metabolic process
GO:0010106 cellular response to iron ion starvation
GO:0019543 propionate catabolic process
GO:0033993 response to lipid
GO:0042594 response to starvation
GO:0046327 glycerol biosynthetic process from pyruvate
GO:0052572 response to host immune response
GO:0071333 cellular response to glucose stimulus
Cellular Component
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0009274 peptidoglycan-based cell wall

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4rcg, PDBe:4rcg, PDBj:4rcg
PDBsum4rcg
PubMed25798914
UniProtP9WIH3|PCKG_MYCTU Phosphoenolpyruvate carboxykinase [GTP] (Gene Name=pckG)

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