Structure of PDB 4qzh Chain A

Receptor sequence

>4qzhA (length=337) Species: 10090 (Mus musculus)

KISQYACQRRTTLNNYNQLFTDALDILAENDELRENEGSCLAFMRASSVL
KSLPFPITSMKDTEGIPCLGDKVKSIIEGIIEDGESSEAKAVLNDERYKS
FKLFTSVFGVGLKTAEKWFRMGFRTLSKIQSDKSLRFTQMQKAGFLYYED
LVSCVNRPEAEAVSMLVKEAVVTFLPDALVTMTGGFRRGKMTGHDVDFLI
TSPEATEDEEQQLLHKVTDFWKQQGLLLYCDILESQKCFLILKLDHGRVH
SGKGWKAIRVDLVMCPYDRRAFALLGWTGSRQFERDLRRYATHERKMMLD
NHALYDRTKRVFLEAESEEEIFAHLGLDYIEPWERNA

3D structure

• PDB: 4qzh Structural basis for a novel mechanism of DNA bridging and alignment in eukaryotic DSB DNA repair.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D343 D345 D434
• Catalytic site (residue number reindexed from 1): D195 D197 D261
• Enzyme Commision number: 2.7.7.31: DNA nucleotidylexotransferase.
  3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	G186 S187 A190 C216 G218 K220 V221	G38 S39 A42 C68 G70 K72 V73
BS02	dna	A	L189 R193 R454 R458 R461 H466	L41 R45 R281 R285 R288 H293
BS03	dna	A	G257 G259 K261 T262 H342 F405 R432	G109 G111 K113 T114 H194 F239 R259
BS04	MG	A	D343 D434	D195 D261
BS05	MG	A	D343 D345	D195 D197
BS06	DCT	A	G332 G333 R336 K338 H342 D343 D345 G449 W450 G452 R454	G184 G185 R188 K190 H194 D195 D197 G276 W277 G279 R281

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4qzh, PDBe:4qzh, PDBj:4qzh
• PDBsum: 4qzh
• PubMed: 25762590
• UniProt: P09838|TDT_MOUSE DNA nucleotidylexotransferase (Gene Name=Dntt)